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Identifier |
000454253 |
Title |
Μελέτη της απόκρισης φυτών Νicotiana benthamiana με δυσλειτουργικό το miRNA μονοπάτι της σίγησης, παρουσία ιών αγρονομικού ενδιαφέροντος |
Alternative Title |
Study of the response of Νicotiana benthamiana plants with a dysfunctional miRNA silencing pathway to agronomically important viruses |
Author
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Μάττος, Ανδρέας-Στυλιανός Α.
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Thesis advisor
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Καλαντίδης, Κρίτων
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Reviewer
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Κοτζαμπάσης, Κυριάκος
Τσαγρή, Ευθυμία
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Abstract |
Plants are subjected to a wide range of stresses that limit crop growth, development,
and productivity. Therefore, plants have developed sophisticated defence mechanisms,
a typical example of which is the RNA silencing, a sequence-specific biological process
conserved in eukaryotes. RNA silencing regulates gene expression at transcriptional
and post-transcriptional level through small RNAs (sRNAs) that play an important role
in the organism's developmental strategy, the maintenance of genome integrity and
plant defence against viruses. The SERRATE multifunctional protein is a key factor in
the silencing mechanism, as it ensures the fidelity of miRNA processing at the
endogenous pathway. Its importance for plants is highlighted by its involvement in
other biochemical pathways and the pleiotropic phenotype of se mutants. According to
literature, the SERRATE protein is associated with plant response to pathogen attacks
such as fungi and bacteria. At the same time, viroid infections of plants with partial
suppression of the SERRATE indicate the importance of the miRNA pathway for plant
defence. Among the biotic stresses that plants are subjected to, viruses are considered
particularly harmful since they can lead to crop productivity reduction, post-harvest
deterioration of fruits and vegetables as well as the destruction of agronomic crops.
In this context, the aim of the present study was to delve into the function of the miRNA
pathway of the RNA silencing, the host-pathogen interactions, and the changes of virus
accumulation in plants with dysfunctional characteristics. For this reason, the genetic
materials of wild-type Nicotiana Benthamiana plants and transgenic plants (SE 5.1i)
with a dysfunctional endogenous RNA silencing pathway were studied in the presence
of cucumber mosaic virus (CMV), tomato spotted wilt virus (TSWV) and turnip mosaic
virus (TuMV). As evaluation parameters for the present study, we used the viral titer
calculation in the plant cells as well as the phenotype control compared between the
two treatments.
Overall, the results of the study demonstrated changes in the accumulation of the
phytopathogenic virus CMV throughout the sampling , the most characteristic of which
was the virus reduction 28 days after the mechanical infections in transgenic plants with
partial suppression of the SERRATE gene compared to wild-type plants. Regarding
TSWV infections, an increasing trend of viral load was observed in the transgenic
plants, while TuMV infected plants were studied only at phenotypic level. Regarding
the symptomatology, no differences were found among the studied genotypes in terms
of the appearance, range and intensity of the symptoms during the three separate
experiments.
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Language |
Greek |
Subject |
Biotic stress |
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CMV |
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RNA silencing |
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RNA σίγηση |
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SERRATE |
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TSWV |
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TuMV |
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Viruses |
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Βιοτικό στρες |
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Ιοί |
Issue date |
2023-03-24 |
Collection
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School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses
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Type of Work--Post-graduate theses
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Permanent Link |
https://elocus.lib.uoc.gr//dlib/8/6/e/metadata-dlib-1678435543-643180-7444.tkl
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Views |
473 |