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Identifier |
000456740 |
Title |
Βιοτεχνολογική καταπολέμηση του δάκου της ελιάς (Bactrocera oleae) με το βακτήριο Wolbachia |
Alternative Title |
Wolbachia as a tool for biotecnological control of Bactrocera oleae |
Author
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Μουστάκα, Ιουλιέττα Μ.
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Thesis advisor
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Βόντας, Ιωάννης
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Reviewer
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Κοτζαμπάσης, Κυριάκος
Καλαντίδης, Κρίτων
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Abstract |
Olea europaea is one of the most economically important tree crops in Greece and the
Mediterranean basin that is known for its nutritional importance in the Mediterranean diet.
The olive fruit fly, Bactrocera oleae, is considered the most serious pest of olives. Its
endosymbiotic bacteria Erwinia dacicola is required by B. oleae in order to complete its
development in unripe green olives. Several control strategies have been used against B.
oleae such as chemical insecticide treatments, sterile insect technique (SIT) and the use of
traps and pheromones.
The purpose of this work was to investigate the potential of Wolbachia, another
obligatory endosymbiotic bacterium, which has already been used in biotechnological
control applications of several insects, to control B. oleae. Specifically, I set two different
goals, firstly, the investigation of the possible interaction between Wolbachia with Erwinia
dacicola, and secondly, the ability of Wolbachia to induce cytoplacmic incompatibility (CI) in
its hosts.
Firstly, we confirmed the maternal transmission of Erwinia and then we localized the
bacteria in the esophageal bulb of adults with PCR, fluorescence microscopy and confocal
microscopy and we estimated the number of bacteria per bulb at 2.5 million with qPCR.
Subsequently we investigated means for the infection of the lab populations with Erwinia
dacicola. We found that the injections of the esophageal bulb containing E. dacicola to the
head of uninfected females is optimal. We created a population with both E. dacicola and
Wolbachia in order to οbserve any possible interactions. Simultaneously, we searched out
for new Wolbachia populations adapted to the enviroment of Crete, which will be ideal for
the application of the control of B. oleae in Crete. We identified two different Wolbachia
strains, the wRi and wMel, and we proceeded in the creation of new lab populations with
these two Wolbachia strains, but without identifying any bacteria transmission.
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Language |
Greek |
Issue date |
2017-03-17 |
Collection
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School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses
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Type of Work--Post-graduate theses
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Permanent Link |
https://elocus.lib.uoc.gr//dlib/5/e/d/metadata-dlib-1687947493-956632-32255.tkl
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Views |
964 |