Results - Details

Search command : Author="Κουτσολέλος"  And Author="Αθανάσιος"

Current Record: 33 of 125

[Add to Basket]
Title	Σύνδεση και χαρακτηρισμός υδατοδιαλυτών πορφυρινικών παραγώγων με εφαρμογή στη φθορίζουσα σήμανση πρωτεϊνών
Author	Μιχαήλ, Παναγιώτα
Thesis advisor	Κουτσολέλος, Αθανάσιος
Abstract	Fluorescent labeling is called the labeling with fluorescent tags, which is used for the detection of the biomolecules, in order to monitor them, to clarify their structure, and the reactions they catalyze. Proteins are considered to be the most widespread and multidimensional macromolecules. In order to detect them, other fluorescent proteins can be used and chemically bind to a particular region in the target-molecule. There are several linking ways between chromophores and biomolecules, hence there are various types of fluorescent labeling, such as protein labeling, enzyme labeling, chemical labeling and genetic labeling. The most commonly used method in fluorescent labeling, is the method for which is used metallochelate coupling that binds chromophores and the amino acids of the protein, which is analyzed. The widely used fluorophores are small organic molecules. Thus far, there are no reports regarding the usage of porphyrin derivatives as fluorophores. In order to broaden the study which is related with the usage of porphyrinoid fluorophores in fluorescent labeling through metallochelate coupling, we synthesized the Tripyridyl-porphyrin-lysine-tricarboxy for coupling with a peptide carrying a histidine sequence via Ni+2. The identification of the compound was performed with mass spectrometry (Maldi-TOF) and with nuclear magnetic resonance spectroscopy (NMR).
Language	Greek
Issue date	2019-07-24
Collection	School/Department--School of Sciences and Engineering--Department of Chemistry--Graduate theses
	Type of Work--Graduate theses
Permanent Link	https://elocus.lib.uoc.gr//dlib/7/9/3/metadata-dlib-1564481056-387540-18015.tkl
Views	186

Digital Documents
	Download document View document Views : 11