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Identifier |
000412519 |
Title |
Λειτουργική ανάλυση της συνεισφοράς της ανθεκτικότητας δύο μεταλλαγών του τασεοευαίσθητου καναλιού νατρίου ενάντια στα εντομοκτόνα indoxacarb και metaflumizone με τη χρήση της τεχνική CRISPR-Cas9 στη Drosophila melanogaster |
Alternative Title |
Functional validation of the contribution of two sodium channel target site mutations in sodium channel blocker insecticides resistance by CRISPR-Cas9 genome modification in Drosophila melanogaster |
Author
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Σαμαντσίδης, Γεώργιος-Ραφαήλ Κ.
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Thesis advisor
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Βόντας, Ιωάννης
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Reviewer
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Δελιδάκης, Χρήστος
Δούρης, Βασίλης
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Abstract |
Insecticide resistance emergence have rendered laborious the counteract of agricultural pests and
vector borne diseases the last decades. Plutella xylostella and Tuta absoluta comprise two major
agricultural pests having a tremendous potential to develop resistance against insecticides such as
Sodium Channel Blocker Insecticides (SCBIs, Indoxacarb and Metaflumizone). Two amino acid
alterations, F1845Y and V1848I (P.xylostella numbering), in the sixth segment of the forth domain of
sodium channel have been correlated with high levels of indoxacarb and metaflumizone resistance.
Although electrophysiological studies have shown the correlation of those mutations with resistance
against SCBIs, no in vivo experimental data have been recorded so far. In this study we have managed
to introduce F1845Y and V1848I mutations in Drosophila melanogaster sodium channel gene
(paralytic), with the CRISPR-Cas9 genome editing technique, in order to investigate functionally the
contribution to SCBIs resistance. F1845Y and V1848I mutations seem to confer higher resistance to
metaflumizone (3460X and 9.3X respectively) than to indoxacarb (12.5X and 6.6X respectively).
Moreover we had the question why F1845Y and V1848I mutations have never been found in the same
allele in field populations of P.xylostella and T.absoluta. For this reason we tried to introduce both
mutations in cis, in order to investigate whether the phenotype is lethal or not. These results provided
useful information about the contribution of those mutations to indoxacarb and metaflumizone
resistance and suggest that both indoxacarb and metaflumizone exhibit stronger binding affinity to the
1845Y than to 1848I.
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Language |
English |
Subject |
Ανθεκτικότητα |
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Δροσόφιλα |
Issue date |
2017-11-22 |
Collection
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School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses
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Type of Work--Post-graduate theses
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Permanent Link |
https://elocus.lib.uoc.gr//dlib/6/e/1/metadata-dlib-1511261550-178569-17437.tkl
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Views |
263 |