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Identifier |
000402455 |
Title |
Study of self-assembling peptides that contain aromatic amino acids |
Alternative Title |
Μελέτη αυτοοργάνωσης πεπτιδίων τα οποία περιέχουν αρωματικά αμινοξέα |
Author
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Ορνιθοπούλου, Ειρήνη
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Thesis advisor
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Μητράκη, Άννα
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Reviewer
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Βαμβακάκη, Μαρία
Πετράτος, Κυριάκος
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Abstract |
Peptides are promising building blocks for tissue engineering applications. In the current
thesis, an amyloidogenic peptide sequence –inspired by the Adenovirus fiber shaft- is rationally
designed to carry photo-structuring and cell-adhesive components. For photo-structuring, aromatic
amino acids that have been grafted on to the N- or C- terminus are: Tyrosine, Tryptophan,
Phenylalanine and Histidine. For cell adhesion the RGD tripeptide has been positioned on the N
terminus. The RGD is known to mimic Fibronectin’s activity in the Extracellular Matrix as a cell
adhesion agent. Electron Microscopy, Atomic Force Microscopy, X-ray Fiber Diffraction and Congo
red staining were used to establish the structure of the produced gels, which definitely showed the
unaffected ability of these designed peptides to build amyloid fibrous networks. For Photo
structuring a KrF pulsed laser has been used, along with the Laser-Induced Fluorescence technique
to examine the production of photo-oxidized moieties, and possible crosslinks. Preliminary results,
using mouse 3T3 fibroblasts show a preference of the cells to adhere and proliferate on the
irradiated surface; therefore, these materials can be candidates for tissue engineering applications.
Finally, an RGD- and Histidine containing peptide is shown to reduce its gelation time exponentially when treated with ultrasound.
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Language |
English, Greek |
Subject |
Tissue engineering |
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Ιστοτεχνολογία |
Issue date |
2016-07-22 |
Collection
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School/Department--School of Sciences and Engineering--Department of Materials Science and Technology--Post-graduate theses
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Type of Work--Post-graduate theses
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Permanent Link |
https://elocus.lib.uoc.gr//dlib/6/3/9/metadata-dlib-1469608003-537000-20657.tkl
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Views |
299 |