|
Identifier |
000453897 |
Title |
Διερεύνηση μολυσματικών πρωτεϊνών φυτικών παθογόνων μέσω δομικών και λειτουργικών προσεγγίσεων |
Alternative Title |
Investigating plant pathogens' virulence proteins through structural and functional approaches |
Author
|
Κωτσαρίδης, Κωνσταντίνος Ι
|
Thesis advisor
|
Σαρρής, Παναγιώτης
|
Reviewer
|
Καλαντίδης, Κρίτων
Μόσχου, Παναγιώτης
Κοτζαμπάσης, Κυριάκος
Χατζηνικολάου, Δημήτριος
Τραντάς, Εμμανουήλ
Παυλίδης, Ιωάννης
|
Abstract |
Plant-microbe molecular interactions is an important research field, the understanding
of which can lead to the design of more successful new strategies for sustainable food
production. The scientific community has considered the structural approach of
protein-protein interactions between a pathogen and its host targets to a limited extent.
However, significant progress has been made in this area due to the rapid
development of artificial intelligence programs capable of predicting the tertiary
structure of a protein and, as a result, further deepening and generating hypotheses on
the functional characterization of proteins.
The general reasoning and experimental design of this thesis is presented in Chapter
One: Introduction, where the basic theory for the conservation of the protein
structures of effectors having similar functions within the host cell is described. A
first application of the tertiary structure calculation and correlation programs to the
function of potential infectious protein factors and their ability to induce programmed
cell death by two strains (Temecula1, CoDiRo) and a subspecies (Sandyi Ann-1) of
the plant pathogen Xylella fastidiosa.
In addition, this PhD thesis presents the results from the structural analyses and
functional characterizations of the type 3 secretion system effectors XopP and RipE1
of the pathogens Xanthomonas campestris pv. campestris and Ralstonia
solanacearum respectively, in the subunit Exo70B1 of the exocytosis complex of the
plant host Arabidopsis thaliana. The XopP effector, as deduced from in vivo and in
vitro analyses, exhibits kinase function, whereas the RipE1 effector, previously
characterized as a cysteine protease, has the ability to cleave the Exo70B1 protein in
vitro.
Overall, this thesis combines a number of techniques and methods from different
areas of biological science (bioinformatics, molecular biology techniques, structural
biology techniques, biophysics/biochemistry assays) and presents an experimental
design for the functional characterization of protein molecules.
|
Language |
Greek |
Subject |
Exo70B1 subunit |
|
Exocyst complex |
|
Functional characterization |
|
In silico prediction of tertiary structure |
|
In silico υπολογισμός τριτοταγής δομή |
|
Type 3 secretion system effectors |
|
Λειτουργικός χαρακτηρισμός |
|
Σύμπλοκο εξωκύττωσης |
|
Τελεστές εκκριτικού συστήματος 3 |
|
Υπομονάδα Exo70B1α |
Issue date |
2023-02-22 |
Collection
|
School/Department--School of Sciences and Engineering--Department of Biology--Doctoral theses
|
|
Type of Work--Doctoral theses
|
Permanent Link |
https://elocus.lib.uoc.gr//dlib/d/0/d/metadata-dlib-1676555478-566761-32319.tkl
|
Views |
811 |