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Identifier

000390212

Title

Study of the Tnfα allele homologous pairing in murine macrophages

Alternative Title

Μελέτη του μηχανισμού ομόλογης αλληλεπίδρασης των Τnfa αλληλίων σε μακροφάγα ποντικού

Author

Στρατήγη, Καλλιόπη

Thesis advisor

Σπηλιανάκης, Χ. Γ.

Abstract

Several trans-allelic phenomena have been described in the past although their mode of action on gene regulation lacks mechanistic insight. Such a rare phenomenon in mammals is somatic homologous pairing. We have investigated the homologous pairing of the Tnfα locus in LPS stimulated mouse macrophages and found that it occurs early upon activation prior to the Tnfα gene expression switch from monoallelic to biallelic. The proposed regulatory mechanism involves two complementary long non-coding RNAs transcribed from the LT/TNF locus, a protein kinase with transactivation potential and a protein considered to be the mammalian homolog of the Drosophila GAGA-factor involved in transvection. We believe that homologous pairing as a part of genome organization in mammals will be of general interest and propose a mechanism for the regulation of mono- to bi-allelic switch of gene expression.

Language

English

Subject

PKM2

ThPOK

lncRNA

Ομόλογη αλληλεπίδραση

Issue date

2015-03-05

Collection

School/Department--School of Sciences and Engineering--Department of Biology--Doctoral theses

Type of Work--Doctoral theses