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Identifier	000381636
Title	The role of SATB1 in long-range chromosomal interactions in murine CD4+ T cells
Alternative Title	Ο ρόλος της SATB1 στις χρωμοσωμικές αλληλεπιδράσεις των CD4+ T κυττάρων
Author	Ψυλλάκη, Κλεάνθη
Thesis advisor	Σπηλιανάκης, Χ
Abstract	Upon antigen encounter, naive CD4+ T cells can differentiate into two major and functionally distinct subpopulations; T helper 1 (Th1) and T helper 2 (Th2). Several epigenetic phenomena regulate the differentiation of naive CD4 cells into the Th1 and Th2 cell lineages. Special AT-rich binding protein 1 (SATB1) plays a crucial role in the establishment and promotion of the epigenetic changes. SATB1 organizes chromatin architecture by tethering distant genomic loci into its network facilitating their coordinated regulation. In thymocyte nuclei SATB1 forms a cage as it is excluded from heterochromatin regions. Here, we provide evidence for the in vivo binding of murine SATB1 in gene loci involved in CD4+ cell lineage commitment. These cytokine gene loci interchromosomally interact in the nucleus of naive CD4 cells and this interaction poises chromatin for transcription. Moreover, by performing rapid amplification of cDNA ends (RACE) experiments, we have identified the different SATB1 transcripts which are expressed in peripheral CD4+ cells and we show their distinct expression, in terms of their mRNA levels, during differentiation. Rabbit polyclonal antisera against a unique SATB1 transcript carrying an extra exon, revealed its protein expression in different CD4+ cell populations. Our findings provide insight into the role of SATB1 in transcriptional regulation during CD4+ T cell differentiation and support the hypothesis for its crucial role in the establishment of epigenetic mechanisms that regulate the CD4+ T cell fate.
Language	English
Issue date	2013-11-15
Collection	School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses
	Type of Work--Post-graduate theses
Permanent Link	https://elocus.lib.uoc.gr//dlib/7/9/e/metadata-dlib-1387540050-988741-22718.tkl
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