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Identifier

000452161

Title

Study of transcription circuits and enhancers involved in neural stem cell fate in Drosophila

Alternative Title

Μελέτη μεταγραφικών κυκλωμάτων και ενισχυτών που ενέχονται στη γένεση νευρικών βλαστικών κυττάρων στη Drosophila

Author

Μπούμπα, Βασιλική Κ.

Thesis advisor

Δελιδάκης, Χρήστος
Θεοδώρου, Βασιλική

Reviewer

Παυλόπουλος, Αναστάσιος
Μοναστηριώτη, Μαρία

Abstract

The nervous system of Drosophila consists of the central and peripheral nervous systems. Neurogenesis is regulated by proneural genes (acute-scute complex: acute, scute, lethal of scute and asense), which drive cells to a neural fate, and neurogenic genes (Notch signalling and Enhancer of split complex), which drive to the epidermal fate. Neuroblasts, the stem cells of the nervous system, divide asymmetrically, resulting in a new neuroblast and a ganglion mother cell (GMC). The GMC divides to produce neurons and glia. During the development of an organism, the genome gives rise to different cell types, each expressing a subset of genes that define its identity. Important in this process are enhancers, which are cis-regulatory elements and determine transcription in each cell type. Their study is important as they bind transcription factors and cofactors and drive gene transcription to desired levels and not to basal level, which results from promoters. In this thesis, transcriptional circuits and enhancers were studied, involved in the embryonic neural stem cells fate, as well as their dependence on proneural genes was examined. Specifically, the enhancers and the genes they control were studied in the wild type and in deficiency environment lacking the three proneural genes except from asense. The results showed that some enhancers and genes are completely dependent on the absent proneural transcription factors, while other enhancers and genes respond in the presence of asense and are activated. Regarding the transcriptional circuits, the ability of deadpan, a transcriptional repressor and neuroblast marker, to repress neural fate repressors, one of the genes of the Enhancer of split complex, was investigated. The results show that deadpan does not suppress the neurogenic gene studied. Deeper study is needed both on the activity of the enhancers, and on the action of deadpan and the transcriptional circuits involved in the neuroblasts fate.

Language

English

Subject

Nervous system

Neuroblast

Ενισχυτές

Νευρικό σύστημα

Νευροβλάστης

Issue date

2022-11-25

Collection

School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses