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Title |
Επίδραση των συνθηκών ανάπτυξης στην παραγωγή εξωπολυσακχαριτών από το φωτοσυνθετικό κυανοβακτήριο Nostoc calcicola |
Alternative Title |
Effects of growth conditions on the production of exopolysaccharides in the photosynthetic cyanobacterium Nostoc calcicola |
Author
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Χατζηαθανασίου, Αγγελική
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Thesis advisor
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Γανωτάκης, Δημήτριος
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Abstract |
In the present work, the effect of growth conditions on the production of
exopolysaccharides by photosynthetic microorganisms was investigated.
The experiment was divided in two parts: In the first one, the microorganism
was cultivated without air inflow under stress conditions for the cells, which
was achieved by removing nitrogen from the growth medium. Also, the
effect of a carbon source, like sodium carbonate, carbon dioxide, glucose
and sodium acetate, on the production of exopolysaccharides by the
cyanobacterium Nostoc calcicola was investigated, due to the absence of any
other carbon source in the medium.
In the second part of the experiment, EPS production with air inflow was
studied in four different growth conditions, in the presence of all
components, in the absence of nitrogen, in the absence of both nitrogen and
carbon, and in the absence of sulfur. Finally, in these conditions, the cells
were studied by optical microscopy.
In the aforementioned cultivations, the cell growth (concentration of
chlorophyll a and dry weight), the concentration of exopolysaccharides and
the quantity of peptides in the supernatant fluid of the cultures were
measured at regular intervals.
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Language |
Greek |
Subject |
Cyanobacteria |
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Exopolysaccharides (EPS) |
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Photosynthetic microorganisms |
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Εξωπολυσακχαρίτες (EPS) |
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Κυανοβακτήρια |
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Φωτοσυνθετικοί μικροοργανισμοί |
Issue date |
2020-07-31 |
Collection
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School/Department--School of Sciences and Engineering--Department of Chemistry--Graduate theses
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Type of Work--Graduate theses
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Permanent Link |
https://elocus.lib.uoc.gr//dlib/1/6/c/metadata-dlib-1594623781-222318-6620.tkl
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Views |
381 |