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Identifier

000388705

Title

Ανίχνευση του ιού human papillomavirus (HPV)στον καρκίνο του πνεύμονα

Alternative Title

Detection of human papillomavirus in lung cancer

Author

Σαρχιανάκη, Εμμανουέλα

Thesis advisor

Σουρβίνος, Γ.

Reviewer

Σπαντίδος, Δ.
Σταθόπουλος, Ε.
Τζανάκης, Ν.
Κουτσόπουλος, Α.
Αντωνίου, Α.
Ζαφειρόπουλο, Α.

Abstract

Although the role of human papillomavirus (HPV) in the development of uterine cervical cancer is well established, the role of HPV in lung carcinogenesis remains controversial. The detection rates of HPV DNA are subject to a wide variation from 0 to 100 %. This is partly influenced by the detection techniques employed. To elucidate the impact of HPV infection on lung parenchyma, we analyzed 100 non small cell lung cancer (NSCLC) specimen (39 squamous cell carcinoma, 50 adenocarcinomas, 5 samples with characteristics of both squamous cell and adenocarcinoma, 5 undifferentiated and 1 large cell carcinoma) from the region of Crete, Greece. Sixteen non cancerous samples served as the negative controls. DNA was extracted from 100 paraffin embedded tissue sections obtained from NSCLC patients. The specimens were examined for the detection of HPV DNA by Real Time PCR using GP5+/GP6+ primers. Furthermore, the HPV positive samples were subjected to genotyping. In contrast to the absence of viral genomes in the control samples, HPV DNA was detected in 19 NSCLC specimens (19%). In particular, 4 squamous cell carcinomas, 12 adenocarcinomas, 1 sample with characteristics of both squamous cell and adenocarcinoma and 2 undifferentiated samples were HPV positive. The distribution of HPV genotypes was as follows: HPV 16:8 cases (42.1 %), HPV 11: 3 cases (15.8 %), HPV 6 : 1 case (5.2 %), HPV 33 : 2 cases (10.5 %), HPV 31 : 2 cases (10.5 %) and HPV 18 : 2 cases (10.5 %). The presence of HPV in the tumor samples provides evidence of the potential role of HPV in NSCLC and strongly argues for additional research on this issue.

Language

English

Subject

lung cancer

Ιός ανθρωπίνων θηλωμάτων

Καρκίνος πνεύμονα

Issue date

2014-12-04

Collection

School/Department--School of Medicine--Department of Medicine--Doctoral theses

Type of Work--Doctoral theses

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