Results - Details
Search command : Author="Κατερινόπουλος"
And Author="Χαράλαμπος"
Current Record: 6 of 79
|
Identifier |
000441863 |
Title |
Παρασκευή παραγώγων της 7-διαιθυλάμινοκουμαρίνης και μελέτη της φθορισμομετρικής τους συμπεριφοράς ως φθορίζοντες δείκτες κατιόντων |
Alternative Title |
Preparation of 7-diethylaminocoumarin derivatives and study of their fluorescent properties as cation fluorescent probes |
Author
|
Ατζαμόγλου, Θεανώ Κ.
|
Thesis advisor
|
Κατερινόπουλος, Χαράλαμπος
|
Reviewer
|
Μήλιος, Κωνσταντίνος
Απόστολος, Σπύρος
|
Abstract |
The synthesis of Mn2+ ion-selective markers has been a long time challenge for the
research community, given the difficulty of the construction of cation chelator systems that
would be selective for the specific target ion.
In the context of the present study, the synthesis of a small series of fluorescent markersderivatives of 7-dimethylaminocoumarin was performed, followed by the study of their
fluorescence profile. It was decided that (a) the fluorophore would contain the chromophore
group of coumarin, while (b) the ionophore would alternatively contain either a carboxyl group,
therefore the probe would contain two carboxyl units (one in the form of the coumarin lactone)
or two pyridine groups, in an attempt to imitate the dipyridine system of the Liang and Canary
probes which were the only indicators that were reported as selective for Mn2+ ions
The markers that were synthesized and studied are (a) 7-(dimethylamino)coumarin (1d),
(b) 4-carboxy-7-(dimethylamino)coumarin (1bc-ONα) and (c) 7-(dimethylamino)-2-oxo-N,Nbis(pyridin-2-ylmethyl)-2H-chromo-3-carboxamide (4c)
All indicators exhibited a "turn-off" response, meaning that the increase of ion
concentration leads to a decrease in the relative fluorescence intensity. Kd values are in the
micromolar (mM) range.
Fluorescence profile studies showed that the presence of the di-(2-picolyl)amine group
contributes to the selectivity for Mn2+ ions, which was the main goal of the research project.
Speciation studies indicate that the 4c / Mn2+ ratio is most likely 2:1, i.e. the system behaves as a
tetradentate chelator complex. Ion competition studies have shown that acetonitrile solutions
have a distinct selectivity for Mn2+ ions.
In conclusion, although fluorescence indicator 4c cannot function as a Mn2+ indicator in
biological (aqueous) systems, it can act as an analytical tool for the determination of Mn2+ levels
in acetonitrile analytical samples.
|
Language |
Greek |
Subject |
Cations |
|
Coumarins |
|
Fluorescent probes |
|
Fluorometric studies |
|
Δείκτες φθορισμού |
|
Κατιόντα |
|
Κουμαρίνες |
|
Φθορισμομετρική μελέτη |
Issue date |
2021-11-24 |
Collection
|
School/Department--School of Sciences and Engineering--Department of Chemistry--Post-graduate theses
|
|
Type of Work--Post-graduate theses
|
Permanent Link |
https://elocus.lib.uoc.gr//dlib/4/5/6/metadata-dlib-1630409162-871915-17630.tkl
|
Views |
260 |