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Identifier

000426718

Title

Διερεύνηση του ρόλου RNase III ενδονουκλεασών στο φυτό N. benthamiana, παρουσία ιοειδούς

Alternative Title

Investigating the role of RNase III endonucleases in N. benthamiana

Author

Καλλέμη, Παρασκευή Ζ.

Thesis advisor

Καλαντίδης, Κρίτων

Abstract

The super – family of RNAse III endonucleases consists of four distinct classes. They are all characterized by the presence of a highly conserved 9 – amino acid stretch in their catalytic centre, namely RNase III signature motif, and their role is the cleavage of double stranded RNA molecules. Viroids of Pospiviroidae family replicate in the nucleus following an asymmetric rolling circle mechanism. During their replication, there is the need of an RNAse III endonuclease action, that would cleave the double stranded RNA concatemer intermediates. In this research, we focus on studying the putative impact of RNAse Three – Like (RTL) proteins on the life cycle of Potato spindle tuber viroid (PSTVd, Pospiviroidae). Specifically, we are trying to functionally characterize two RTL proteins (RTLA and RTLB) of Nicotiana benthamiana plants, upon PSTVd infection, based on the Virus Induced Gene Silencing (VIGS) assay. Our findings indicate the defensive role of RTLA and the possible implication of RTLB in viroid’ s replication or movement in plants.

Language

Greek

Subject

PSTVD

RTL

Viroids

Ιοειδή

Issue date

2019-11-29

Collection

Faculty/Department--Faculty of Sciences and Engineering--Department of Biology--Post-graduate theses

Type of Work--Post-graduate theses