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Identifier 000385942
Title Ανάπτυξη πειραματικού αυτεμβολίου έναντι παθογόνων βακτηρίων σε εκτρεφόμενα λαβράκια (Dicentrarchus labrax)
Alternative Title Development of experimental autogenus vaccine against bacteria pathogenic to intensively cultured seabass (Dicentrarchus labrax)
Author Σμυρλή, Μαρία Ν.
Thesis advisor Παυλίδης, Μιχάλης
Reviewer Μυλωνάς, Κωνσταντίνος
Καθάριος, Παντελής
Abstract The purpose of this study was the characterization and identification of a bacterial pathogen causing mortality and morbidity in cultured sea bass (Dicentrarchus labrax), initially identified as Aeromonas sp. and the development of an experimental autogenous vaccine to confront the related disease. The particular disease occurs regularly in recent years and affects fish of commercial size. The bacterial strains that were isolated from diseased seabass were identified and characterized by phenotypic markers and molecular tools. The causative agent of the disease was determined as an Aeromonas veronii strain, a species not previously reported in sea bass. One of the strains was used for the preparation of an experimental autogenous vaccine and infectivity, response to antibiotics and optimal growth conditions were further studied. An inactivated bacterin vaccine was prepared and intraperitoneally injected to seabass fish in concentrations of 107 and 109 cells ml-1. The immune response of the vaccinated fish was studied based on serum lysozyme activity and specific antibody titer for 75 days post-vaccination. Antibody titer was detected in both bacterin concentrations at dilutions ranging from 1:1000-1:8000. Significant differences compared to the control group (unvaccinated fish), were detected between days 14th to 44th in bacterin concentration 109 cells ml-1, a time period for which, antibody title remains constant and may be compared with adjuvanted vaccines. There was no change in the concentration of lysozyme. The results of this work suggest that immunity can be achieved as long as the vaccine is administered at least two weeks before the expected outbreak of the disease, in bacterin concentration of 109 cells ml-1. However, a challenge experiment is necessary in order to assess the vaccine’s efficacy of protection (relative percent survival-RPS).
Language Greek
Subject Aquaculture
Issue date 2014-07-25
Collection   Faculty/Department--Faculty of Sciences and Engineering--Department of Biology--Post-graduate theses
  Type of Work--Post-graduate theses
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