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Identifier |
000449906 |
Title |
Η διερεύνηση του ρόλου και των λειτουργικών και μοριακών χαρακτηριστικών των μεσεγχυματικών κυττάρων (MSCs) στην ενδομυελική ήλωση |
Alternative Title |
The investigation of the role and the functional and molecular characteristics of MSCs in the intramedullary nailing |
Author
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Σπερελάκης, Ιωάννης
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Thesis advisor
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Κοντάκης, Γεώργιος
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Reviewer
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Αντωνίου, Αικατερίνη
Δημητρίου, Ελένη
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Abstract |
Reamed intramedullary nailing (RIN) is a surgical method of choice for treatment of diaphyseal fractures. This procedure affects the biological environment of bone tissue locally and systemically. This study investigated the influence of RIN on mesenchymal stem cells (MSCs) in patients with long bone fractures. The axis of C-X-C motif chemokine receptor 4 (CXCR 4)/stromal cell-derived factor 1 (SDF‑1) was selected since it is considered as major pathway for MSC homing and migration.
Iliac crest bone marrow (IC‑ BM) samples and blood samples were collected at two different time points. One sample was collected before the RIN (BN) and the other immediately after RIN (AN). BM‑MSCs were cultured and RT‑q PCR was performed for CXCR4 mRNA levels and ELISA for the SDF‑1 sera levels.
The experimental study revealed that there was a correlation between the increase of SDF‑1 levels in peripheral blood and a decrease in the levels of CXCR 4 in MSCs in the IC‑ BM following RIN .The levels of SDF‑1 showed a significant increase in the sera of patients after RIN. In conclusion, the present study is the first providing evidence of the effects of RIN on MSC population via the CXCR 4/SDF‑1 axis. The levels of serum SDF‑1 factor were elevated after RIN while increased levels of SDF‑1 in peripheral blood were inversely correlated with the mRNA levels of CXCR 4 on BM‑MSCs after RIN.
Therefore, this study contributes to enlighten the systematic effects of RIN on the population of MSCs at a cellular level.
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Language |
Greek |
Subject |
AXIS SDF-1/CXCR4 |
Issue date |
2022-07-29 |
Collection
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School/Department--School of Medicine--Department of Medicine--Doctoral theses
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Type of Work--Doctoral theses
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Permanent Link |
https://elocus.lib.uoc.gr//dlib/c/c/2/metadata-dlib-1658129104-849005-30510.tkl
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Views |
292 |
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