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Title Μελέτη ανάπτυξης του βακτηρίου Bradyrhizobium sp. για την καθήλωση N2 και παραγωγή H2
Alternative Title A study of the growth, N2 fixation and H2 production by the bacterium Bradyrhizobium sp.
Author Σταμοβλάση – Σπυροπούλου, Φλωρεντία
Thesis advisor Γανωτάκης, Δημήτριος
Abstract In this thesis, a series of culture conditions were studied to produce biological hydrogen from an isolated strain of Bradyrhizobium sp. Factors related to the composition of the nutrient medium and how they affect hydrogen production and nitrogen fixation by the bacterium were studied. The effect of the concentration of the metals Fe2+ and Mg2+, CaCO3, NaCl and the concentration of sucrose, which is the source of organic carbon in the nutrient medium, was investigated. In addition, alternative carbon sources besides sucrose were used, such as different sugars and other organic substrates. Finally, the effect of the nitrogen source and degassing gas on nitrogen fixation and hydrogen production was investigated. The aim of the above experiments was to find the conditions under which the bacterium Bradyrhizobium sp. produces the maximum possible amount of hydrogen, but also investigating the relationship of biological hydrogen production with nitrogen fixation and carbon source metabolism. From the study of the above, it was found that the production of hydrogen gas by the bacterium Bradyrhizobium sp. it is mainly linked to the metabolism of the sugar contained in the growth medium, and the optimal conditions to produce the gas were determined. The production of hydrogen by the specific bacterium of the genus Bradyrhizobium is the subject of the patent application that has already been filed to H.I.P.A with case number 2413-0004708944 by the laboratory's research team.
Language Greek
Subject Biological hydrogen
Hydrogen
Nitrogen fixation
Nitrogen fixing bacteria
Sugars metabolism
Αζωτοδεσμευτικά βακτήρια
Βιολογικό υδρογόνο
Καθήλωση αζώτου
Μεταβολισμός σακχάρων
Υδρογόνο
Issue date 2023-07-27
Collection   School/Department--School of Sciences and Engineering--Department of Chemistry--Graduate theses
  Type of Work--Graduate theses
Permanent Link https://elocus.lib.uoc.gr//dlib/b/b/2/metadata-dlib-1687505118-163212-7742.tkl Bookmark and Share
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