Your browser does not support JavaScript!

Home    Search  

Results - Details

Search command : Author="Γραβάνης"  And Author="Αχιλλέας"

Current Record: 8 of 83

Back to Results Previous page
Next page
Add to Basket
[Add to Basket]
Identifier 000450954
Title Biomaterial-based drug delivery in CNS injuries
Alternative Title Μεταφορά φαρμάκων μέσω βιουλικών σε τραύματα νευρικού συστήματος
Author Παπαγιαννάκη, Αργυρή
Thesis advisor Γραβάνης, Αχιλλέας
Reviewer Χαραλαμπόπουλος, Ιωάννης
Τζεράνης, Δημήτριος
Abstract Central nervous system (CNS) injuries, including Spinal Cord Injury (SCI) and Optic Nerve Crush (ONC), are major health problems worldwide with high morbidity and mortality, affecting thousands of individuals each year. Spinal cord injury (SCI) may be defined as an injury resulting from an insult inflicted on the spinal cord that compromises, either completely or incompletely, its major functions (motor, sensory, autonomic, and reflex). Traumatic optic neuropathy (TON) is a condition, induced when an injury occurs within the optic pathway and results in vision impairment or permanent vision loss. Despite significant efforts to unravel the mechanisms that underlie wound healing response after CNS injury, existing treatments are still deficient. There is an urgent need to understand better the pathophysiology of CNS injury and develop efficient therapeutic strategies. The study aims to develop novel SCI and/or ONC therapies that combine 3D biomaterials (porous collagen scaffolds, PCS), small molecule analogs of neurotrophins (microneurotrophins, MNT) and neural stem cells (NSCs). Initially, evaluate the characteristics (duration, amount) of MNT delivery via PCS using a drug delivery system comprised of MNT entrapment in peptide gels inside PCS. Secondly, evaluate MNT neuroprotective and neurogenic effects in neural cells by quantifying cell phenotypes related to SCI and/or ONC, namely Neural Stem Cell proliferation, and differentiation. The aim of this project is to characterize and optimize the delivery of MNTs in vitro and provide promising neuroimplants that can be further evaluated in established SCI/ONC animal models. MNT delivery was quantified using model drugs. Fluorescein was entrapped inside a porous collagen-GAG scaffold, and inside a porous collagen-GAG scaffold in a gel formed by self- assembled peptides for release kinetics assays. The release assay results demonstrate a sustained delivery of about a 6-hour duration, while the peptide does not seem to delay the release from the PCS further. In vitro release was studied in neural stem cell culture with brdU delivery. The release was successfully translated in a cell phenotype alteration and the entire quantity of the molecule was released. Furthermore, BBN27 effects on NSCs proliferation were studied in a 2D cell culture and differentiation in a 3D cell culture. BNN27 did not seem to have any effect on NSC proliferation. Finally, regarding differentiation BNN27 seemed to induce a small increase on NSC differentiation towards neuron lineage even though no significance was detected by statistical analysis
Language English, Greek
Subject Collagen scaffold
Microneurotrophins
Neural stem cells
Ικρίωμα κολλαγόνου
Μικρονευροτροφίνες
Νευρικά βλαστικά κύτταρα
Issue date 2022-07-29
Collection   School/Department--School of Medicine--Department of Medicine--Post-graduate theses
  Type of Work--Post-graduate theses
Permanent Link https://elocus.lib.uoc.gr//dlib/2/c/1/metadata-dlib-1663683208-420206-1142.tkl Bookmark and Share
Views 339

Digital Documents
No preview available

No permission to view document.
It won't be available until: 2025-07-29