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Identifier

000411955

Title

Non-Linear microscopy applications, for imaging of sub-cellular structures & tissue samples

Alternative Title

Εφαρμογές της μη γραμμικής μικροσκοπίας, για την απεικόνιση υποκυτταρικών δομών και ιστών

Author

Αρχοντάκης Εμμανουήλ Ε.

Thesis advisor

Χαραλαμπίδης, Δημήτριος

Reviewer

Φιλιππίδης, Γιώργος

Abstract

In this interdisciplinary study, the activation of mouse T-cells was investigated by using Third Harmonic Generation (THG) microscopy as a diagnostic tool. Previous studies shown, that the main intracellular structures that provide high THG signals are lipid depositions. The aim of this work was to develop a system, where quantification of recorded THG signals could provide information vis-à-vis the activation state of T-cells. Thus, qualitative and quantitative analysis of resting and activated T-cells was performed by using THG microscopy. Based on the quantification of the collected THG signals it was depicted, that activated T-cells present increased lipid depositions and average surface area in comparison with the normal ones. Two different ways of T-cell activation was achieved: a) with mitogen Concanavalin-A, and b)with specific human albumin antigen (HSA). In addition, the quantification of our measurementsrevealed that the amount of lipid depositions and the average cell surface upon the activation Con-A, is greater in comparison to the activation with HSA. The obtained results are very encouraging for the potential employment of THG imaging modality, as a non-destructive tool for cancer immunodiagnostics, as it allows following-up of immune response development, and for the reliable screening of the action of immunosuppressive drugs. Finally, in the framework of this interdisciplinary study, a limited number of THG imaging measurements were performed to normal and cancer breast biopsy samples.

Language

English

Subject

Biophysics

Breast Tissues

Cancer immunodiagnostics

Nonlinear optics

T-cells

TPEF

Third-harmonic generation

Βιο-φυσική

Βιοψία μαστού