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Identifier |
000377737 |
Title |
Η ανίχνευση του ιού του ανθρώπινου θηλώματος (HPV) σε ασθενείς με καρκίνο ουροδόχου κύστεως με τη μέθοδο της ποσοτικής αλυσιδωτής αντίδρασης με πολυμεράαση (real-time quantitative PCR) |
Alternative Title |
The detection of human papilloma virus (HPV) in patients with urinary bladder cancer using a quantitative polymerase chain reaction (real-time quantitative PCR) technique |
Author
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Παναγιωτάκης, Γεώργιος Ι
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Thesis advisor
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Σπαντίδος, Δημήτριος Α
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Reviewer
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Σουρβίνος, Γεώργιος
Τσατσάνης, Χρήστος
Ζώρας, Οδυσσέας
Τσατσάκης, Αριστείδης
Ζαφειρόπουλος, Α.
Μαμουλάκης, Χ.
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Abstract |
The aim of the present PhD thesis was to estimate the detection of
human papillomavirus (HPV) in tumourigenesis of human urinary bladder,
using the Real Time quantitative polymerase chain reaction (Real-Time
qPCR) and the effectiveness of this method, in order to be a future safe
protocol for the detection of HPV in patients with bladder cancer. Furthermore
we studied the detection of human herpesviruses and Polyoma viruses (BK
and JC) in the same patients, using the method of polymerase chain reaction.
For this PhD thesis we used 30 specimens of tumour tissue and 30
specimens of adjacent healthy bladder tissue, which originated from patients
who underwent surgical transurethral resection at the Department of Urology,
Asklipeiion General Hospital, Voula, Athens, Greece.
We failed to detect positive samples for HPV infection, not only from
cancerous bladder specimens, but also from adjacent healthy bladder tissue,
although we used three different methods for detection of genetic material of
the virus, with progressively increasing sensitivity (PCR, nested-PCR, RTqPCR)
We obtained different results trying to detect human herpesvirus and
Polyoma virus genome, using the polymerase chain reaction (PCR)
technique. For HSV-1, HSV-2, VZV and HHV-7 both tumour and normal
specimens, were negative for the detection of the presence of viral specific
genome.
Cytomegalovirus DNA was detected in 22 tumour samples and 23
normal samples. 17 paired samples were also detected for CMV DNA.
EBV DNA was detected in 15 tumour samples and 4 normal samples.
1 paired sample was also detected for EBV DNA.
HHV-6 DNA was detected in 11 tumour samples and 1 normal sample.
3 paired samples were also detected for HHV-6 DNA.
KSHV DNA was detected in 1 tumour sample and 2 normal samples.
None paired sample was detected for KSHV DNA.
Polyoma BK and JC viral DNA was detected in 4 tumour samples.
None normal sample was positive for the detection of Polyoma BK and JC
viral DNA. None paired sample was detected for Polyoma BK and JC viral
DNA.
Meanwhile EBV DNA presence was abundant in tumour samples
compared to normal tissue with statistical significance (p=0,0048). A small yet
notable percentage of samples examined (10%) contained CMV and HHV-6
(6,7%), CMV and EBV (3,3%) sequences raising the possibility of synergism
between these viruses in urinary bladder carcinogenesis.
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Language |
Greek |
Subject |
HPV |
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Real time quantitative PCR |
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Urinary Bladder Neoplasms |
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Καρκίνος ουροδόχου κύστεως |
Issue date |
2012-12-11 |
Collection
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School/Department--School of Medicine--Department of Medicine--Doctoral theses
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Type of Work--Doctoral theses
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Permanent Link |
https://elocus.lib.uoc.gr//dlib/8/5/6/metadata-dlib-1356682370-306848-17498.tkl
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Views |
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