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Identifier 000453897
Title Διερεύνηση μολυσματικών πρωτεϊνών φυτικών παθογόνων μέσω δομικών και λειτουργικών προσεγγίσεων
Alternative Title Investigating plant pathogens' virulence proteins through structural and functional approaches
Author Κωτσαρίδης, Κωνσταντίνος Ι
Thesis advisor Σαρρής, Παναγιώτης
Reviewer Καλαντίδης, Κρίτων
Μόσχου, Παναγιώτης
Κοτζαμπάσης, Κυριάκος
Χατζηνικολάου, Δημήτριος
Τραντάς, Εμμανουήλ
Παυλίδης, Ιωάννης
Abstract Plant-microbe molecular interactions is an important research field, the understanding of which can lead to the design of more successful new strategies for sustainable food production. The scientific community has considered the structural approach of protein-protein interactions between a pathogen and its host targets to a limited extent. However, significant progress has been made in this area due to the rapid development of artificial intelligence programs capable of predicting the tertiary structure of a protein and, as a result, further deepening and generating hypotheses on the functional characterization of proteins. The general reasoning and experimental design of this thesis is presented in Chapter One: Introduction, where the basic theory for the conservation of the protein structures of effectors having similar functions within the host cell is described. A first application of the tertiary structure calculation and correlation programs to the function of potential infectious protein factors and their ability to induce programmed cell death by two strains (Temecula1, CoDiRo) and a subspecies (Sandyi Ann-1) of the plant pathogen Xylella fastidiosa. In addition, this PhD thesis presents the results from the structural analyses and functional characterizations of the type 3 secretion system effectors XopP and RipE1 of the pathogens Xanthomonas campestris pv. campestris and Ralstonia solanacearum respectively, in the subunit Exo70B1 of the exocytosis complex of the plant host Arabidopsis thaliana. The XopP effector, as deduced from in vivo and in vitro analyses, exhibits kinase function, whereas the RipE1 effector, previously characterized as a cysteine protease, has the ability to cleave the Exo70B1 protein in vitro. Overall, this thesis combines a number of techniques and methods from different areas of biological science (bioinformatics, molecular biology techniques, structural biology techniques, biophysics/biochemistry assays) and presents an experimental design for the functional characterization of protein molecules.
Language Greek
Subject Exo70B1 subunit
Exocyst complex
Functional characterization
In silico prediction of tertiary structure
In silico υπολογισμός τριτοταγής δομή
Type 3 secretion system effectors
Λειτουργικός χαρακτηρισμός
Σύμπλοκο εξωκύττωσης
Τελεστές εκκριτικού συστήματος 3
Υπομονάδα Exo70B1α
Issue date 2023-02-22
Collection   School/Department--School of Sciences and Engineering--Department of Biology--Doctoral theses
  Type of Work--Doctoral theses
Permanent Link https://elocus.lib.uoc.gr//dlib/d/0/d/metadata-dlib-1676555478-566761-32319.tkl Bookmark and Share
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