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Identifier 000454253
Title Μελέτη της απόκρισης φυτών Νicotiana benthamiana με δυσλειτουργικό το miRNA μονοπάτι της σίγησης, παρουσία ιών αγρονομικού ενδιαφέροντος
Alternative Title Study of the response of Νicotiana benthamiana plants with a dysfunctional miRNA silencing pathway to agronomically important viruses
Author Μάττος, Ανδρέας-Στυλιανός Α.
Thesis advisor Καλαντίδης, Κρίτων
Reviewer Κοτζαμπάσης, Κυριάκος
Τσαγρή, Ευθυμία
Abstract Plants are subjected to a wide range of stresses that limit crop growth, development, and productivity. Therefore, plants have developed sophisticated defence mechanisms, a typical example of which is the RNA silencing, a sequence-specific biological process conserved in eukaryotes. RNA silencing regulates gene expression at transcriptional and post-transcriptional level through small RNAs (sRNAs) that play an important role in the organism's developmental strategy, the maintenance of genome integrity and plant defence against viruses. The SERRATE multifunctional protein is a key factor in the silencing mechanism, as it ensures the fidelity of miRNA processing at the endogenous pathway. Its importance for plants is highlighted by its involvement in other biochemical pathways and the pleiotropic phenotype of se mutants. According to literature, the SERRATE protein is associated with plant response to pathogen attacks such as fungi and bacteria. At the same time, viroid infections of plants with partial suppression of the SERRATE indicate the importance of the miRNA pathway for plant defence. Among the biotic stresses that plants are subjected to, viruses are considered particularly harmful since they can lead to crop productivity reduction, post-harvest deterioration of fruits and vegetables as well as the destruction of agronomic crops. In this context, the aim of the present study was to delve into the function of the miRNA pathway of the RNA silencing, the host-pathogen interactions, and the changes of virus accumulation in plants with dysfunctional characteristics. For this reason, the genetic materials of wild-type Nicotiana Benthamiana plants and transgenic plants (SE 5.1i) with a dysfunctional endogenous RNA silencing pathway were studied in the presence of cucumber mosaic virus (CMV), tomato spotted wilt virus (TSWV) and turnip mosaic virus (TuMV). As evaluation parameters for the present study, we used the viral titer calculation in the plant cells as well as the phenotype control compared between the two treatments. Overall, the results of the study demonstrated changes in the accumulation of the phytopathogenic virus CMV throughout the sampling , the most characteristic of which was the virus reduction 28 days after the mechanical infections in transgenic plants with partial suppression of the SERRATE gene compared to wild-type plants. Regarding TSWV infections, an increasing trend of viral load was observed in the transgenic plants, while TuMV infected plants were studied only at phenotypic level. Regarding the symptomatology, no differences were found among the studied genotypes in terms of the appearance, range and intensity of the symptoms during the three separate experiments.
Language Greek
Subject Biotic stress
CMV
RNA silencing
RNA σίγηση
SERRATE
TSWV
TuMV
Viruses
Βιοτικό στρες
Ιοί
Issue date 2023-03-24
Collection   School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses
  Type of Work--Post-graduate theses
Permanent Link https://elocus.lib.uoc.gr//dlib/8/6/e/metadata-dlib-1678435543-643180-7444.tkl Bookmark and Share
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