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Identifier

000426711

Title

Investigating putative subcellular targets of Ralstonia solanacearum type III effectors

Alternative Title

Αναζήτηση των πιθανών υποκυτταρικών στόχων παραγόντων παθογένειας (effectors) του παθογόνου Ralstonia solanacearum

Author

Τσακίρη, Δήμητρα Ν.

Thesis advisor

Σαρρής, Παναγιώτης

Reviewer

Καλαντίδης, Κρίτωνας
Μόσχου, Παναγιώτης

Abstract

In a world with an ever-growing population and an ever-changing climate, sustainable agriculture and the pathogens that threaten it are crucial for food security. Ralstonia solanacearum is an aggressive Gram-negative bacterium with a wide host range, which includes plant species of both monocots and dicots. Its pathogenicity mostly depends on the Type III Secretion System (T3SS) delivery machine. The effector proteins secreted from the T3SS into the host cell can interfere with various eukaryotic cell processes, including immunity responses. NLRs (Nucleotide-binding Leucine-rich repeats Receptors) are plant intracellular immunity receptors, that activate plant defense, upon effector recognition. The Integrated Decoy or ID model describes NLR domains serving as baits, that seem to have derived from the original subcellular target of the effector. In this study, we used yeast to hybrid screening in order to identify the interactions between pathogen effectors and Decoy domains. A screening for interactions between pathogen effectors and a list of frequently appearing IDs could uncover the effectors’ putative original targets. We tested 6 effectors from the soil-borne bacterial pathogen Ralstonia solanacearum against 23 Integrated Decoys deriving from plant NLRs and we identified 11 putative interactions. Understanding such interplay on a protein level can give insight to virulence mechanisms of bacterial wilt disease and provide novel targets for genome editing approaches, that introduce resistance.

Language

English

Subject

Effectors

NLR

Phytopathogen

Ralstonia

Virulence

Τελεστές

Υποδοχείς άμυνας

Φυτοπαθογόνο

Issue date