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Identifier |
000358621 |
Title |
In vitro μελέτη της AtERI-LIKE1 : καθαρισμός, απομόνωση και προσπάθεια ανεύρεσης του φυσικού υποστρώματός της. |
Alternative Title |
In vitro study of AtERI-LIKE1: overexpression, purificationa and effort to reveal its natural substrate |
Author
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Βλατάκης, Ιωάννης Χ
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Thesis advisor
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Κρίτωνας, Καλαντίδης
Πανόπουλος, Νικόλαος
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Abstract |
The protein superfamily of DEDD exonucleases consists of several members, which
fulfill variant and important functions in RNA metabolism in a large spectrum of
organisms. From participating in pathways of tRNA and rRNA processing and maturation in
bacteria, to participation in DNA polymerase complexes as proofreading subunits,
maturation of rRNAs and in regulation of RNAi pathways, DEDD exonucleases have
attracted strong scientific interest over the last years. This interest is focused on their
action. Their preference for double stranded RNA molecules that contain single stranded
3’ ends as substrates seems to dictate their function. The interest around a subgroup of
this family, the so called Eris, which participate in rRNA and siRNA pathways (even though
assumptions have been made for their participation in other processes such as the
maturation of 3’ end of histone mRNAs), boosted scientific effort towards the
characterization of their members in an increasing number of organisms. This dissertation
is a part of this effort and its main goal was to characterize the ΕRI member, AtERI‐LIKE1
from Arabidopsis thaliana, which is the first member ever found to be localized into the
chloroplasts. In a series of in vitro experiments AtERI‐LIKE1 was tested against a number of
possible substrates (ssRNA, dsRNAi‐like, 5 S rRNA, 5,8 S rRNA) to determine whether it
could bind to or process them. The results did not definitively show interaction between
ΑtERI‐LIKE1 and the tested substrates. However, western blotting with anti‐AtERI‐LIKE1
serum against protein extracts from plant tissues indicated that more than one isoform of
ΑtERI‐LIKE1 may be expressed in Arabidopsis thaliana plants.
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Language |
Greek |
Subject |
3' εξωνουκλεάσες |
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3-5 exonuclease |
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DEDD superfamily |
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EXOIII |
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Υπεροικογένεια πρωτεινών DEDD |
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μετα-μεταγραφική ρύθμιση RNA |
Issue date |
2010-07-16 |
Collection
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School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses
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Type of Work--Post-graduate theses
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Permanent Link |
https://elocus.lib.uoc.gr//dlib/9/9/d/metadata-dlib-d9abf920e103cc7cec957f2494fc1db7_1279191251.tkl
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Views |
228 |