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Identifier	000418848
Title	Development of an imaging platform for in-vivo observations in mouse models using combined photoacoustic and optical microscopy
Alternative Title	Ανάπτυξη απεικονιστικής πλατφόρμας για in-vivo μετρήσεις σε μοντέλα ποντικών συνδυάζοντας φωτοακουστική και οπτική μικροσκοπία
Author	Νταλόπουλος, Αντώνης Β.
Thesis advisor	Χαραλαμπίδης, Δημήτριος Ζαχαράκης, Γιάννης
Reviewer	Παπάζογλου Δημήτρης
Abstract	Photoacoustic microscopy (PAM) is a novel in vivo imaging modality whose contrast arises from the detection of acoustic waves generated through the photoacoustic (PA) arises from the detection of acoustic waves generated through the photoacoustic (PA) effect. According to the PA effect, light is absorbed by molecules and converted into heat; this causes a local thermoelastic expansion of the medium leading to the generation of an acoustic wave which is subsequently detected using an ultrasonic transducer. In this work we have developed an imaging platform for label-free, in-vivo observations in mouse models, through the integration of PAM and confocal fluorescence microscopy (CFM) into a single instrument in order to provide complementary contrast modes. More specifically, PAM could image ear vasculature due to the high absorption of visible radiation by hemoglobin, whereas CFM recorded the intrinsic autofluorescence of skin tissue originating predominantly from elastin and collagen. For these in-vivo measurements we have used laboratory mice (BALB/c) which were anaesthetized using a mixture of isoflurane with oxygen supply. Mice were subsequently placed on a custom-made platform dedicated for the imaging of the ear region, which is beneficial for imaging due to its small thickness. The information obtained by the recorded co-registered images could be used for the study of several pathological conditions such as cancer and metabolic disorders, drug delivery processes etc. establishing thus a powerful hybrid imaging protocol applied in fundamental biomedical research.
Language	English
Issue date	2018-11-23
Collection	School/Department--School of Sciences and Engineering--Department of Physics--Post-graduate theses
	Type of Work--Post-graduate theses
Permanent Link	https://elocus.lib.uoc.gr//dlib/d/0/0/metadata-dlib-1540274144-385460-18640.tkl
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