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Identifier

000389804

Title

Μελέτη των προγονικών ενδοθηλιακών κυττάρων σε νέους ασθενείς με σακχαρώδη διαβήτη τύπου Ι

Alternative Title

Study of endothelial progenitor cells in young patients with diabetes mellitus Type I

Author

Σημαντηράκη, Δέσποινα Ε.

Thesis advisor

Τσιλιμπάρης, Μιλτιάδης

Reviewer

Παπαδάκη, Ελένη
Γανωτάκης, Εμμανουήλ

Abstract

Purpose: To evaluate the presence of Endothelial Progenitor Cells (EPCs) in the peripheral blood of young patients with Diabetes Mellitus Type I (T1DM). Method & Material: Blood samples were collected and analyzed from 69 individuals (mean ageyears: 27, range:8-50): 39 patients with DM (mean DM duration-years: 16 (range:2-31)), mean age-years: 26(8-50) and 30 controls without DM mean age-years: 28 (range: 9-45). The presence of Diabetic Retinopathy (DR) was documented with slit lamp examination and fluorescence angiography, according to the Early Treatment of Diabetic Retinopathy Study (ETDRS) criteria. The EPCs were detected with flow cytometry using the following primary antibodies: anti-CD34, anti-CD45, anti-CD133 and anti-hVEGFR2. Results: Progenitor EPCs, defined as CD45-/CD34+/CD133+/VEGFR2+ cells, were detected in 31/69 samples (mean%: 0.00042, SEM:0.00007). 20/39 patients had EPCs detected in the periphery blood (mean%: 0.00054, SEM:0.00014). EPCs were detected in 11/15 patients with PDR (mean%: 0.00061, SEM:0.00013). Also, 4/9 patients with NPDR (mean%: 0.00071, SEM:0.00039) and 5/15 patients without clinically proven DR (mean%: 0.00038, SEM:0.00026) had detectable EPCs. Finally, circulating EPCs were identified in 11/30 individuals of the control group (mean%: 0.00026, SEM:0.00007). Conclusions: CD45-/CD34+/CD133+/hVEGFR2+ EPCs were detected in the peripheral blood of non diabetic persons and diabetic patients with or without evidence of DR. The presence of EPCs in the peripheral blood of young diabetics seem to correlate with the DR stage. Study of EPCs in these patients could offer significant information about the pathophysiology of diabetic microangiopathy.

Language

Greek

Subject

Diabetes Mellitus Type I

Endothelial progenitor cells

Προγονικά ενδοθυλιακά κύτταρα

Σακχαρώδης Διαβήτης Τύπου Ι

Issue date

2015-03-31

Collection

School/Department--School of Medicine--Department of Medicine--Post-graduate theses

Type of Work--Post-graduate theses