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Identifier

000393123

Title

Οξειδωτική βλάβη του DNA στο άσθμα

Alternative Title

Oxidative DNA Damage in Asthma

Author

Πρόκλου, Αθανασία Κ.

Thesis advisor

Σιαφάκας, Ν.

Reviewer

Σχίζα, Σ.
Σουρβίνος, Γ.
Σαμώνης, Γ.
Σπαντίδος, Δ.
Τσατσάνης, Χ.
Αντωνίου, Χ.

Abstract

Background: Lung cytotoxic mechanisms trigger the release of perforin and granzymes, causing oxidative DNA damage that ultimately leads to apoptosis. These effects, although demonstrated in COPD, have not been investigated in patients with asthma and in particular in patients with asthma who smoke. Our aim was to measure perforin, granzyme A, granzyme B, and 8-OHdG expression in sputum from smoking and nonsmoking patients with asthma, compared with smoking and nonsmoking control subjects. Methods: Perforin, granzyme A, granzyme B, and 8-OHdG expression levels were detected by enzyme-linked immunosorbent assays (ELISA) in induced sputum specimens. Results: Perforin expression was increased in 40% of smokers and 45% of smoking patients with asthma and in only 7% of nonsmoking patients with asthma (p=0,004), compared with control subjects’ values. In contrast, granzymes A and B levels were increased in 40% of patients in all three groups vs control subjects. Finally, 8-OHdG levels were elevated in 35% of smoking patients with asthma, in 20% of smokers, and in only 10% of nonsmoking patients with asthma. Statistical analysis revealed a positive correlation between granzyme A (p=0,001) and granzyme B (p= 0,006) expression levels and the number of pack-years in smoking patients with asthma. Conclusions: Asthma cytotoxic immune response is mainly represented by granzymes A and B, whereas in smoking patients with asthma perforin and 8-OHdG are additionally involved, resembling the immune response in COPD.

Language

Greek

Subject

Cytotoxicity

Perforin

Γκρανζυμών Α,Β,8-OHdG

Περφορίνη

Issue date

2015-03-31

Collection

School/Department--School of Medicine--Department of Medicine--Doctoral theses

Type of Work--Doctoral theses