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Identifier

000388620

Title

Acoustic sensors for protein and cell binding

Alternative Title

Ακουστικοί αισθητήρες για μελέτη πρόσδεσης πρωτεϊνών και κυττάρων

Author

Χρονάκη, Δήμητρα Ι.

Thesis advisor

Γκιζελή, Ηλέκτρα

Reviewer

Αθανασάκη, Ειρήνη
Χαλεπάκης, Γιώργος

Abstract

In this study we gained a better insight on protein adsorption and cell adhesion processes using the acoustic biosensor Quartz Crystal Microbalance with Dissipation monitoring (QCM-D). Specifically, real-time adsorption of three extracellular proteins was monitored on Au surfaces. The proteins tested were collagen, fibronectin and fibrinogen and their adsorption resulted in significant information regarding their orientation on Au. For results interpretation we used the “discrete model” theory, which is a molecular approach of the adsorbed layer rather than the usual film approach. Acoustic ratio measurements (ΔD/ΔF) were combined with Sauerbrey equation for monolayer coverage to predict orientation of adsorbed molecules. Furthermore, cell adhesion studies were performed for two cell lines: normal (Nthy) and malignant (ARO) thyroid cells. QCM-D technique was recruited to distinguish dynamic cell adhesion signatures of the two cell lines. QCM-D frequency and dissipation shifts were accompanied by optical microscopy images of the adhered cells. Binding isotherms proved the concentration-dependent nature of cell adhesion process and report a different binding capacity of Nthy and ARO on Au. Finally, cell adhesion kinetics revealed remarkable differences of two cell lines on the underlying substrate.

Language

English

Subject

Cell adhesion

Kinetics

Protein adsorption

Κινητική

Προσκόλληση κυττάρων

Προσρόφηση πρωτεϊνών

Issue date

2014-11-21

Collection