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Identifier

000449819

Title

Κλωνοποίηση, έκφραση και χαρακτηρισμός αντιγονικών πρωτεϊνών του παθογόνου Coxiella burnetii

Alternative Title

Cloning, expression and characterization of antigenic proteins of the pathogen Coxiella burnetii

Author

Γιαπιντζάκη, Αφροδίτη Γ.

Thesis advisor

Τσιώτης, Γεώργιος

Reviewer

Παυλίδης, Ιωάννης
Ψαρουλάκη, Άννα

Abstract

Coxiella burnetii is an obligate intracellular bacterium that causes Q fever in humans and other mammals. Q fever is a worldwide zoonotic disease and can cause acute or chronic life- threatening disease in humans. Acute Q fever is mostly a mild flu-like illness. In a percentage of patients infected with C. burnetii, the primary infection will be developed into chronic Q fever. Chronic Q fever leads to high mortality rates if left untreated, making preventive measures critical for high-risk patients. Q fever has become a serious public health problem in many areas, not previously known as endemic areas and poses a significant risk to human and animal health. Therefore, the reliable diagnosis of the disease is essential. In the present study, a number of C. burnetii antigenic proteins were tested for their diagnostic ability in a number of patient sera. Five proteins have been selected that have been described literature-specific for acute Q fever. The plasmids carrying the expression genes of these proteins were constructed and transformed into E. coli cells. The expression conditions for each protein, temperature, inducer concentration and time after induction were then checked, and the optimal ones were determined. Isolation of the proteins was followed, and their antigenicity was studied with serums of patients from acute and chronic Q fever, as well as healthy blood donors. The sensitivity and specificity of these tests were determined by statistical analysis. Based on these results, a protein was selected for immobilization on a gold electrode and studied by electrochemical impedance spectroscopy. This device could later be used as a serological diagnostic tool.

Language

Greek

Subject

Q fever

Serological diagnostic tool

Αντιγονικές πρωτεΐνες

Ορολογικό διαγνωστικό εργαλείο

Πυρετός Q

Issue date

2022-07-28

Collection

School/Department--School of Sciences and Engineering--Department of Chemistry--Post-graduate theses