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Identifier 000403428
Title Μελέτη της επίδρασης της καταστολής της DCL3 στην μεταμεταγραφική σίγηση διαγονιδίου μάρτυρα
Alternative Title Study of the effect of DCL3 downregulation in the post transcription silencing of a reporter gene
Author Μαυροθαλασσίτη, Ελένη Δ.
Thesis advisor Καλαντίδης, Κρίτων
Reviewer Καμπράνης, Σωτήριος
Τσαγρή, Μίνα
Abstract This study is divided in two parts. The first part is about the “Study of the effect of DCL3 downregulation in the post transcriptional silencing of a reporter gene”. RNA silencing is the sequence-specific suppression of gene expression through the involvement of RNA. Plants use RNA silencing mostly for three purposes: 1) establishment and maintenance of heterochromatin state, 2) regulation of several endogenous genes and 3) antiviral defense. The trigger of antiviral RNA silencing is a double-stranded RNA that can occur during viral replication or in dsRNA structures into the viral genome. The dsRNAs are then processed into short interfering RNAs (siRNAs) and lead the post-transcriptional gene silencing (PTGS) of the viral RNA. Viruses encode for proteins that suppress RNA silencing, enabling the infection and its spread. Viral suppressors can target either the RNA molecules or the proteins and the complexes participating in the silencing pathway. As part of the effort to understand the biology of the silencing mechanism in total, this study addresses the issue of a possible alternative role of the enzyme DCL3, which in the bibliography has been connected to the branch of silencing mechanism that leads to the formation and maintenance of heterochromatin. We investigated the role of this enzyme in the path of post transcriptional gene silencing. Trying to answer this question we investigated the post transcriptional gene silencing of the reporter gene GFP in different genetic backgrounds. The second part of this report is the “Investigation of the effect of the overexpression of the wild type and the mutated DXS enzyme of the plant Salvia pomiferain the terpene production in transgenic Nicotiana benthamiana lines”. This part of the study follows some previous work as part of a Bachelor’s thesis and aims to produce stable plant lines overproducing an improved DXS enzyme.
Language Greek
Subject Plants
Issue date 2016-11-18
Collection   Faculty/Department--Faculty of Sciences and Engineering--Department of Biology--Post-graduate theses
  Type of Work--Post-graduate theses
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