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Identifier 000410083
Title Ανάπτυξη ζωικού μοντέλου οφθαλμικού μελανώματος του χοριοειδούς
Alternative Title Development of an animal model for uveal melanoma
Author Ιωαννίδη Λαρίσα Δ.
Thesis advisor Δετοράκης, Ευστάθιος
Reviewer Τσιλιμπάρης, Μιλτιάδης
Σουρβίνος, Γεώργιος
Abstract PURPOSE: The purpose of our study is to create a repeatable and easy-to-use animal model of choroidal ocular melanoma, which may be used as a research tool in imaging studies and treatment methods. METHODS: Two sets of experiments were conducted. In the first set, 3 albino rabbits weighing 2 - 2.5 kg were used. The animals were subjected to systemic immunosuppression with intramuscular injections of dexamethasone 2 mg / kg, 3 times daily with a 6-hour interval. The process of cell implantation involved the infusion of cells in the anterior chamber – iris of the right (OD) eye for 2 animals (cell lines: A375, 92.1) and the implantation of cells in the anterior chamber - iris and subconjunctival space (12 o’clock area above the sclerocorneal limbus) of the OD eye (cell line Mel202) for 1 animal. In the second set, two albino rabbits weighing 2.5-3 kg were used. The animals were subjected to systemic immunosuppression by intramuscular injections of 15 mg / kg / day CyclosporinA for 3 days prior to the inoculation of melanoma cells and continued to receive the same dose of cyclosporin A for 26 days post-inoculation until the day of sacrifice. The procedure for the Mel202 animal experiment involved the vaccination of Mel202 cells in the anterior chamber of the OD eye. The 92.1 series cells were also used and propagated in the 92.1 experimental OD eye in the supra-choroidal space. During the experiments, all animals were examined every 5 days using a portable slit lamp and indirect ophthalmoscopy. Additionally, MRI with surface coil was performed for the second set of animals, and subsequently all eyes were enucleated and histologically examined. RESULTS: During the first experiment, no abnormal lesion was observed, suggesting the presence of uveal melanoma. Histological images showed the presence of lymphocytes and a possible picture of an inflammatory reaction. During the second experiment, no damage was also noted suggesting the presence of uveal melanoma in the Mel202 animal. Similar results were seen in experimental animal 92.1 but due to the site of cell implantation and the difficulty of observing a potential tumor, an MRI scan using surface coil with a T1 and T2 – weighted images was performed in which the potential lesion of the suspected area was depicted. An histological analysis followed, which confirmed the presence of melanoma in the ciliary body. CONCLUSIONS: In this study, we aimed at creating an animal model of rapid uveal melanoma development, taking into account the smaller size of the developing volume. The challenge in this case is the difficulty of imaging and monitoring such a small tumor with conventional techniques. Finally, by testing the various methods of cell implantation, we concluded that suprachoroidal implantation was successful compared to other pathways. Despite the short duration of the model, we have seen that using the surface coil we can depict the small developing tumor, which especially in the case of the ciliary body cannot be distinguished in any other way because of its size.
Language Greek, English
Issue date 2017
Collection   Faculty/Department--School of Medicine--Department of Medicine--Post-graduate theses
  Type of Work--Post-graduate theses
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