Results - Details
Search command : Author="Βόντας"
And Author="Ιωάννης"
Current Record: 13 of 76
|
Identifier |
000443710 |
Title |
Functional expression and characterization of cytochrome P450s, associated with pesticide toxicity |
Alternative Title |
Λειτουργική έκφραση και χαρακτηρισμός κυτοχρωμικών μονοξειδάσων P450s που συνδέονται με την τοξικότητα που προκαλούν τα παρασιτοκτόνα |
Author
|
Καϊλή, Σταυρούλα Ι.
|
Thesis advisor
|
Βόντας, Ιωάννης
|
Reviewer
|
Παυλίδης, Ιωάννης
Χαλεπάκης, Γεώργιος
|
Abstract |
Ιnsecticide selectivity and resistance is an ongoing challenge concerning both crop pest control
and insect-born diseases control, and it has been largely associated with cytochrome P450
monoοxyganases’ metabolism. Functional characterization of P450s is of great importance, in
order to validate their involvement in insecticide resistance and to develop selective and safe
insecticides (e.g. for bee pollinators) and insecticide synergists. In the present study, two distinct
cases of P450s that have been associated with resistance to insecticides were heterologously
expressed in E. coli for their functional characterization. Firstly, HaCYP6B7, which was previously
found to be associated with fenvalerate metabolism in Helicoverpa armigera, was co-expressed
in E. coli together with Musca domestica CPR (MdCPR). HaCYP6B7 was expressed in its native and
truncated form in the membrane or in the cytoplasm of bacteria, respectively. The recombinant
enzyme was assayed for its catalytic activity against model substrates. In the second part of this
study, the aim was to heterologously express in E. coli BoCYP6G2 and BoCYP6A23 that have been
related with resistance to α-cypermethrin in Bactrocera oleae. The two P450s were successfully
cloned into expressing vectors and BoCYP6G2 proceeded to membrane targeting experiments
together with Bactrocera oleae CPR (BoCPR).
|
Language |
English |
Subject |
Heterologous expression in E.Coli |
|
Ετερόλογη έκφραση στο σύστημα E.Coli |
|
Κυτόχρωμα P450 |
|
Τοξικότητα παρασιτοκτόνων |
Issue date |
2021-11-26 |
Collection
|
School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses
|
|
Type of Work--Post-graduate theses
|
Permanent Link |
https://elocus.lib.uoc.gr//dlib/5/6/b/metadata-dlib-1637062286-159881-21146.tkl
|
Views |
381 |