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Identifier |
000434000 |
Title |
Analysis of insecticide resistance mechanisms in enemies of public health and agriculture with the use of -omics technologies and bioinformatics |
Alternative Title |
Μοριακή ανάλυση ανθεκτικότητας εντόμων υγειονομικής σημασίας και εχθρών καλλιεργειών στα εντομοκτόνα με χρήση ομικών τεχνολογιών και βιοπληροφορικής |
Author
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Χαραμής, Ιάσωνας Μ.
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Thesis advisor
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Βόντας, Ιωάννης
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Reviewer
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Παυλίδης, Παύλος
Νικολάου, Χριστόφορος
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Abstract |
Insecticide resistance imposes great challenges in public health and global food
security. This study focused on investigating mechanisms of insecticide resistance
by using genomic and transcriptomic data. More specifically, we studied the
possible association of gene transcripts expressed in the legs of resistant
mosquitoes with insecticide resistance. The transcriptomic data suggest that the
constitutive resistance phenotype is associated with the over-expression of
cytochrome P450s and other detoxification enzymes, while there is also a possible
contribution of cuticular resistance mechanisms. Moreover, to uncover genes
participating in deltamethrin induction we examined the leg-specific
transcriptomic profile of this highly resistant strain after exposure to deltamethrin.
The results of the transcriptomic analysis suggest that deltamethrin induces the
expression of genes coding for detoxification enzymes and chemosensory proteins
with a possible pyrethroid-protecting role. In addition, it also indicates the
involvement of GPCRs in the development of resistance after exposure to the
insecticide. Further, we studied the evolution of cytochrome P450s in the olive fly
Bactrocera oleae and performed phylogenetic comparisons with the P450omes of
Drosophila melanogaster and Ceratitis capitata. This analysis demonstrated that B.
oleae bears expansions in the CYP12A and CYP12E subfamilies belonging to the
mitochondrial clan and the CYP6A and CYP6G subfamilies belonging to the
CYP3 clan of cytochrome P450s. In summary, this study demonstrates the
complex mechanisms controlling constitutive resistance and deltamethrin
induction in A. coluzzii and has generated a valuable genomic resource for
experimental and computational studies in B. oleae.
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Language |
English |
Subject |
Cytochrome P450 |
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Genomics |
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Malaria vector |
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Olive fruit fly |
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Transcriptomics |
Issue date |
2020-11-27 |
Collection
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School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses
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Type of Work--Post-graduate theses
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Permanent Link |
https://elocus.lib.uoc.gr//dlib/7/5/a/metadata-dlib-1605519763-422804-16773.tkl
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Views |
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