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Identifier

000447739

Title

Establishment of Cretan brain bank : first observations and perspectives

Author

Ταβλαδωράκης, Εμμανουήλ

Thesis advisor

Ζαγανάς, Ιωάννης

Reviewer

Κουτσόπουλος, Αναστάσιος
Χαραλαμπόπουλος, Ιωάννης

Abstract

Dementia, and Alzheimer’s disease in particular, is a leading cause of mortality and morbidity worldwide, and its impact is expected to rise with the aging population in several countries. While research in neurodegenerative diseases, such as AD, is typically administered on cell cultures and animal models, these are not sufficient to fully elucidate the pathophysiology in its entirety, despite their significant contribution to our current understanding of AD and related dementias. The study of postmortem brain tissue from patients stricken by AD and related dementias is crucial for our understanding of disease mechanisms. In this study, we describe the establishment of a brain bank (Cretan Brain Bank; CBB) focused on AD and related dementias in Crete, Greece. CBB builds on an ongoing collaboration between the University of Crete, Medical School, Panhellenic Biobank of Neurological Diseases (PAVINN) and the AD Association of Heraklion (“Allelegye”). Our aim is to advance research on human neurodegeneration using the CBB as a tool. The study was approved by the Ethics Committee of the University Hospital of Heraklion and all subjects and their legal representatives gave informed consent. One hemisphere of the obtained brain is stored in formalin and the other is coronally sliced and stored at -80°C. Formalin-fixed paraffin embedded brain tissue sections are used for immunohistochemical (IHC) staining for Aβ amyloid and pTau protein for neuropathological validation of clinical diagnosis, while frozen hemispheres are utilized for wet biology, including Western-Blots and enzymatic studies. To date, ten of fifteen brains have been histopathologically characterized as AD utilizing IHC for Aβ amyloid and pTau protein on sections from the hippocampal area. Also, immunohistochemical stains from different specific brain regions were employed for the evaluation of the disease stage. These neuropathologically characterized brains will provide a solid basis for further research on neurodegenerative diseases

Language

Greek, English

Subject

Alzheimer disease

Dementia

Human tissue bank

Άνοια

Ανοσοϊστοχημικές χρώσεις

Βιοτράπεζα εγκεφάλου

Γλουταμική αφυδρογονάση

Νόσος Alzheimer

Issue date