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Identifier 000434179
Title Ο ρόλος της RNA σίγησης στην αντίδραση υπερευαισθησίας επαγόμενη από ένα ζεύγος NLR
Alternative Title The role of RNA silencing in an NLR-induced hypersensitivity response
Author Αμαρτωλού, Αργυρούλα Μ.
Thesis advisor Σαρρής, Παναγιώτης
Reviewer Καλαντίδης, Κρίτων
Τσαγρή, Ευθυμία
Abstract The aim of this study was to study the role of RNA silencing in the BnRPR1 / BnRPR2 resistance pair-induced hypersensitivity response. Plants have developed their innate immune system, which has been biochemically differentiated into two pathways. The first pathway, known as the basal innate immune system, recognizes and responds to molecules common to many categories, including non-pathogens. The second, referred to as the immune system of resistance genes (ETIs), responds to infectious agents secreted by pathogens, i.e. the operators, which are recognized by NLRs. NLRs are intracellular receptors and promote innate immunity. Their structure consists of the LRR domain, a NB nucleotide binding domain, and an amino-terminal domain. The regulation of homeostasis NLRs genes are important for their function. In healthy plants, NLR proteins are found in small amounts and / or in an inactive form. The setting of the NLR expression can occur at several levels. One mechanism for regulating NLRs, which we will study in this paper, is that of RNA silencing. Higher eukaryotic organisms have this RNA sequence mechanism, which results in the inhibition of gene expression, either at the transcriptional or translational level. In N. benthamiana plants, both wild-type and those suppressed by DCL2 (dcl2), DCL4 (dcl4) and suppression of DCL2 and DCL4 (dcl2 /4), the hypersensitivity responce and the amount of gene expression BnRPR1 / BnRPR2, after their expression by agro-infiltration will be observed. In this way we will determine which of the proteins DCL2 and DCL4 regulates the expression of the genes under study.
Language Greek
Subject BnRPR1
BnRPR2
DCL proteins
DCL πρωτεΐνες
Plant immune system
SRNA
Ανοσοποιητικό σύστημα
Issue date 2020-11-27
Collection   School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses
  Type of Work--Post-graduate theses
Permanent Link https://elocus.lib.uoc.gr//dlib/0/0/c/metadata-dlib-1606212701-508215-20451.tkl Bookmark and Share
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