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Identifier

000381600

Title

Δημιουργία εργαλείων για την in vivo παρατήρηση μιτοχονδρίων στο νευρικό σύστημα των νηματωδών- ρόλος της MAGE-1 στη γήρανση του C. elegans

Alternative Title

Creating tools for monitoring mitochondria in the nematode nervous system in vivo-role of MAGE-1 in the ageing of C.elegans

Author

Χαρμπίλας, Νικόλαος

Thesis advisor

Ταβερναράκης, Νεκτάριος

Reviewer

Αλεξανδράκη, Δέσποινα
Μαυροθαλασσίτης, Γεώργιος

Abstract

In the current study, we have developed tools for monitoring mitochondria specifically in the neuronal system of the nematode C. elegans in vivo. Furthermore, we examined the possible involvement of protein MAGE-1, the closest homologue of MAGE protein family, in the process of ageing of worms. Overexpression of MAGE-1 under its own promoter or under a neuron-specific one reduces the median lifespan of worms and increases their sensitivity in heat and UV stress. In contrast, knock-down of MAGE-1 expression has no effect in ageing, but increases the activity of transcription factors DAF-16, SKN-1 and autophagy. Neuron-specific genetic inhibition of MAGE-1 leads to morphological abnormalities in neuronal axons, highlighting a tissue-specific role of the protein in maintaining organism’s physiology.

Language

Greek

Subject

Ageing

Mitochondria

Γήρανση

Μιτοχόνδρια

Issue date

2013-11-15

Collection

School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses

Type of Work--Post-graduate theses