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|
Identifier |
000430980 |
Title |
Ανάπτυξη και βελτιστοποίηση βιοδιεργασιών καταλυόμενων από τρανσαμινάσες για τη σύνθεση δομικών λίθων φουρανικών πολυμερών |
Alternative Title |
Development and optimization of bio-processes catalyzed by transaminases for the synthesis of building blocks for furane polymers. |
Author
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Κελεφιώτης Στρατηδάκης, Παναγιώτης Δ.
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Thesis advisor
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Παυλίδης, Ιωάννης
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Reviewer
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Γανωτάκης, Δημήτριος
Σμόνου, Ιουλία
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Abstract |
Αim of this Master's Thesis was the amination of furanoid compounds for the production
of polyamide and polyimine biopolymers. The furoanoid compounds which were used in this
project can be produced by enzymatic or chemical treatment of by-products of agricultural
processes, following the principles of circular economy.
Initially, the catalytic activity of 17 recombinant transaminases from laboratory’s toolbox
was investigated towards the thermodynamically favored deamination reaction of furfurylamine
and 5-methylfurfurylamine in two pH (7.5 and 9.0), while the deamination of 1-phenylethylamine
was investigated as a benchmark substrate. Transaminases from Vibrio fluvialis, Rhodobacter
sphaeroides, Chromobacterium violaceum and Ruegeria pomeroyi gave the most promising
results. Preliminary results on the processes of interest, transaminases from R. sphaeroides
and R. pomeroyi were excluded from further studies, owe to instability issues and low
productivity, respectively. Thus, the next experiments were performed with the transaminases
from V. fluvialis and C. violaceum. The selected enzymes were purified, using affinity
chromatography before applied to desired reactions. For the polyimide polymer, furan-2,5-
dicarvaldehyde was selected, while for the polyamide the 5-formyl-2-furancarboxylic acid, after
the amination. Dicarvaldehyde’s amination leads to spontaneous polymer formation, possibly
due to Schiff base formation at the alkaline reaction conditions. This polymer was analyzed by
Scanning Electron Microscopy (SEM) as well as by Fourier Transform Infrared Spectroscopy
(FTIR). 5-Formyl-2-furancarboxylic acid is also aminated efficiently from both transaminases,
thus a optimization of reaction conditions was performed. It was found that the optimal reaction
conditions with isopropylamine as amine donor was pH 9.0 and 400 mM isopropylamine,
conditions under which the reaction is completed with 100 % conversion under 30 min. In
conclusion, the bioprocesses developed in this work can lead to the synthesis of innovative
biopolymers.
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Language |
Greek |
Subject |
Biocatalysis |
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Biopolymers |
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Chromobacterium violaceum |
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Circular Economy |
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Furan |
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Isopropylamine |
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Vibrio fluvialis |
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Βιοκατάλυση |
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Βιοπολυμερή |
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Ισοπροπυλαμίνη |
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Κυκλική Οικονομία |
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Φουράνιο |
Issue date |
2020-07-31 |
Collection
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School/Department--School of Sciences and Engineering--Department of Chemistry--Post-graduate theses
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Type of Work--Post-graduate theses
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Permanent Link |
https://elocus.lib.uoc.gr//dlib/6/9/3/metadata-dlib-1596097051-357825-3075.tkl
|
Views |
401 |