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Identifier

000416933

Title

Ανοσοδιαγνωστικός προσδιορισμός τοξικών ουσιών σε δείγματα νερού και κρασιού

Alternative Title

Immunodiagnostic determination of toxic substances in water and wine samples

Author

Δασκαλάκη, Σταματία Ε.

Thesis advisor

Ρίζος, Απόστολος

Reviewer

Τσατσάκης, Αριστείδης
Τζατζαράκης, Εμμανουήλ

Abstract

In the present study, a qualitative and quantitative analysis of toxic chemical compounds has been carried out, which can affect human health. We first dealt with the analysis of the active compound Round Up herbicide, glyphosate and secondly with mycotoxin, ochratoxin A. Quantitative and qualitative analysis of the two compounds was done by enzyme immunoassay ELISA. ELISA is a widely used method for both research and diagnostic purposes as it is a method of high selectivity and sensitivity. The glyphosate analysis was performed for wine and water samples, while the analysis of ochratoxin A was performed only on samples of wine. Nine (9) samples of drinking water and twelve (12) samples of wine were analyzed for glyphosate from Crete. The quantitative results ranged from 0.075 to 0.765 ppb for glyphosate in water and from 1.23 to 13.18 ppb for glyphosate in wine. The mean value for water samples was 0.31 ± 0.39 ppb with a median value of 0.09 ppb and 7.09 ± 3.78 ppb with a median value of 7.61 ppb for wine samples. Overall, for glyphosate in water we had 88.9% positive results and 11.1% which had exceeded the legislative limit (EU: 0.1ppb.) For glyphosate in wine we had 100% positive results but none from the samples was not exceed the maximum residue Limit of 0.2 ppm (EPA: 200ppb). A total of one hundred eighty-two samples (182) of wine were analyzed for ochratoxin A, of which one hundred and sixty-four (164) originated from Northern Greece and eighteen (18) came from Crete. The quantitative results ranged from 0.30 to 1.19 ppb for Northern Greece samples with an average of 0.65 ± 0.25 ppb and a median value of 0.63 ppb. For samples from Crete, the results ranged from 0.39 to 0.99 ppb with an average value of 0.69 ± 0.16 ppb and a median value of 0.68 ppb. Overall, we had positive results for ochratoxin A at 47% and 88.9% for wine samples of Northern Greece and Crete respectively. However, no sample of wine exceeded the maximum residue limit of ochratoxin A (2 ppb).

Language

Greek

Subject

Glyphosate

Ochratoxin A

Γλυφοσάτη

Ωχρατοξίνη Α

Issue date

2018-07-18

Collection

School/Department--School of Sciences and Engineering--Department of Chemistry--Post-graduate theses

Type of Work--Post-graduate theses