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Identifier 000412966
Title Α) Στερεοεκλεκτικές βιοκαταλυτικές αναγωγές προς σχηματισμό χειρόμορφων λακταμών Β) Ανάπτυξη και έλεγχος δραστικότητας των ενζύμων Ralstonia ADH και Sphingobium SPP
Alternative Title A) Stereoselective biocatalytic reductions towards the synthesis of chiral lactams B) Development and activity control of the enzymes Ralstonia ADH and Sphingobium SPP
Author Τυρίκος-Εργάς, Θεόδωρος Π.
Thesis advisor Σμόνου, Ιουλία
Reviewer Στρατάκης, Εμμανουήλ
Τσιώτης, Γεώργιος
Abstract The present thesis consists of three Chapters, in which the stereoselective enzymatic reactions using NADPH-dependent alcohol dehydrogenases for the synthesis of valuable chiral intermediates, were studied. Chapter 1 presents the process of developing and isolating recombinant E.coli cells, containing genes encoding two new alcohol dehydrogenases, RasADH and SyADH. The isolated cells were lyophilized and tested for their activity in further experiments. Chapter 2 presents the chemoenzymatic synthesis of the epimer of the natural product (R)-rugulactone. The α-pyrones are inhibitors of the nuclear facton NF-kB associated with various cellular functions. The key step for the synthesis of the main intermediate, is the selective enzymatic reduction of an allyl ester. Finally, Chapter 3 presents the study of the enantioselective reduction of α-nitrile-β-ketoesters using isolated ketoreductases to form optically active, high value intermediates. The -nitrile-β-hydroxyesters can be cyclized to give the corresponding chiral γ-lactams
Language Greek
Subject Biocatalysis
Issue date 2017-11-23
Collection   Faculty/Department--Faculty of Sciences and Engineering--Department of Chemistry--Post-graduate theses
  Type of Work--Post-graduate theses
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