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Identifier |
000433966 |
Title |
Ανάπτυξη βιοαισθητήρα εμπέδησης για κορτιζόλη βασισμένος σε εκτυπωμένα ηλεκτρόδια χρυσού και άνθρακα |
Alternative Title |
Development of impedance biosensor for cortisol based on gold and carbon electrodes |
Author
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Γιαννακάκη, Ευαγγελία Μ.
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Thesis advisor
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Χανιωτάκης, Νικόλαος
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Reviewer
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Γανωτάκης, Δημήτριος
Παυλίδης, Μιχαήλ
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Abstract |
The aim of this thesis was the development of a non-Faradaic impedance biosensor
for cortisol, based on gold and carbon screen printed electrodes. Gold and carbon are
suitable for electrochemical signal transducers due to their unique electrochemical
properties, high conductivity and their ability to immobilize biological element on
their chemically modified electrode surface. Cortisol monoclonal antibody is used as a
biological element due to its high specificity in cortisol. Antibody was covalently
immobilised on the modified electrode surface gold and carbon. The detection of
cortisol by the non-Faradaic biosensor is based on the change in the capacitance and
resistance at the electrode/electrolyte interface, which occurs when a recognition
complex between antibody and cortisol is formated.
For the development of the biosensor, the activity of the antibody was first evaluated
and then, the stages of its construction and the effect of different conditions of the
experimental process were studied. The response of the biosensor to cortisol and
finally, the reproducibility of its construction were examined. The electrochemical
characteristics and monitoring of the biosensor response to cortisol were evaluated by
an electrochemical method, which was the electrochemical impedance spectroscopy.
Τhe results obtained by this study showed that the cortisol biosensor was successfully
fabricated on gold and carbon screen printed electrodes, with the surface of carbon
being the most suitable substrate for developing a cortisol biosensor with greater
reproducibility during manufacturing.
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Language |
Greek |
Subject |
Electrochemical impedance spectroscopy |
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Non-Faradaic biosensor |
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Screen printed electrode |
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Άνθρακας |
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Βιοαισθητήρας εμπέδησης |
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Εκτυπωμένο ηλεκτρόδιο |
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Μη φαρανταϊκός βιοαισθητήρας |
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Φασματοσκοπία ηλεκτροχημικής εμπέδησης |
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Χρυσός |
Issue date |
2020-11-27 |
Collection
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School/Department--School of Sciences and Engineering--Department of Chemistry--Post-graduate theses
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Type of Work--Post-graduate theses
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Permanent Link |
https://elocus.lib.uoc.gr//dlib/f/8/6/metadata-dlib-1605690428-834088-28777.tkl
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Views |
387 |