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Identifier

000433966

Title

Ανάπτυξη βιοαισθητήρα εμπέδησης για κορτιζόλη βασισμένος σε εκτυπωμένα ηλεκτρόδια χρυσού και άνθρακα

Alternative Title

Development of impedance biosensor for cortisol based on gold and carbon electrodes

Author

Γιαννακάκη, Ευαγγελία Μ.

Thesis advisor

Χανιωτάκης, Νικόλαος

Reviewer

Γανωτάκης, Δημήτριος
Παυλίδης, Μιχαήλ

Abstract

The aim of this thesis was the development of a non-Faradaic impedance biosensor for cortisol, based on gold and carbon screen printed electrodes. Gold and carbon are suitable for electrochemical signal transducers due to their unique electrochemical properties, high conductivity and their ability to immobilize biological element on their chemically modified electrode surface. Cortisol monoclonal antibody is used as a biological element due to its high specificity in cortisol. Antibody was covalently immobilised on the modified electrode surface gold and carbon. The detection of cortisol by the non-Faradaic biosensor is based on the change in the capacitance and resistance at the electrode/electrolyte interface, which occurs when a recognition complex between antibody and cortisol is formated. For the development of the biosensor, the activity of the antibody was first evaluated and then, the stages of its construction and the effect of different conditions of the experimental process were studied. The response of the biosensor to cortisol and finally, the reproducibility of its construction were examined. The electrochemical characteristics and monitoring of the biosensor response to cortisol were evaluated by an electrochemical method, which was the electrochemical impedance spectroscopy. Τhe results obtained by this study showed that the cortisol biosensor was successfully fabricated on gold and carbon screen printed electrodes, with the surface of carbon being the most suitable substrate for developing a cortisol biosensor with greater reproducibility during manufacturing.

Language

Greek

Subject

Electrochemical impedance spectroscopy

Non-Faradaic biosensor

Screen printed electrode

Άνθρακας

Βιοαισθητήρας εμπέδησης

Εκτυπωμένο ηλεκτρόδιο

Μη φαρανταϊκός βιοαισθητήρας

Φασματοσκοπία ηλεκτροχημικής εμπέδησης

Χρυσός