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Identifier |
000426718 |
Title |
Διερεύνηση του ρόλου RNase III ενδονουκλεασών στο φυτό N. benthamiana, παρουσία ιοειδούς |
Alternative Title |
Investigating the role of RNase III endonucleases in N. benthamiana |
Author
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Καλλέμη, Παρασκευή Ζ.
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Thesis advisor
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Καλαντίδης, Κρίτων
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Abstract |
The super – family of RNAse III endonucleases consists of four distinct
classes. They are all characterized by the presence of a highly conserved 9 – amino
acid stretch in their catalytic centre, namely RNase III signature motif, and their role
is the cleavage of double stranded RNA molecules. Viroids of Pospiviroidae family
replicate in the nucleus following an asymmetric rolling circle mechanism. During
their replication, there is the need of an RNAse III endonuclease action, that would
cleave the double stranded RNA concatemer intermediates. In this research, we focus
on studying the putative impact of RNAse Three – Like (RTL) proteins on the life
cycle of Potato spindle tuber viroid (PSTVd, Pospiviroidae). Specifically, we are
trying to functionally characterize two RTL proteins (RTLA and RTLB) of Nicotiana
benthamiana plants, upon PSTVd infection, based on the Virus Induced Gene
Silencing (VIGS) assay. Our findings indicate the defensive role of RTLA and the
possible implication of RTLB in viroid’ s replication or movement in plants.
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Language |
Greek |
Subject |
PSTVD |
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RTL |
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Viroids |
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Ιοειδή |
Issue date |
2019-11-29 |
Collection
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School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses
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Type of Work--Post-graduate theses
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Permanent Link |
https://elocus.lib.uoc.gr//dlib/7/a/e/metadata-dlib-1575548345-324480-11306.tkl
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Views |
318 |