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Identifier

000421697

Title

Ανάλυση δειγμάτων από επιφάνειες χρησιμοποιώντας φασματομετρία μάζας με Ιοντισμό/Εκρόφηση Υπερηχητικής Εκνέφωσης

Alternative Title

Analysis of samples from surfaces using Desorption Sonic-Spray Ionization mass spectrometry

Author

Βουγιούκας, Γρηγόριος Σ.

Thesis advisor

Περγαντής, Σπύρος

Reviewer

Κατερινόπουλος, Χαράλαμπος
Σπύρος, Απόστολος

Abstract

In this thesis, the technique of Desorption Sonic-Spray Ionization Mass Spectrometry, DeSSI-MS also known as Easy Ambient Sonic-Spray Ionization Mass Spectrometry (EASI-MS) was developed in the laboratory and applied to three separate sample categories. In the first part, whole coffee beans, of different varieties, were analyzed directly from their surfaces and more than twenty metabolites found in coffee beans were identified. In raw coffee beans, the presence of a class of C-5HTs compounds, which according to bibliographic references are responsible for stomach disorders in susceptible persons, is noteworthy. Ascertained, these compounds are eliminated during barking or roasting of the coffee. Natural metabolites of coffee are triacylglycerols, the presence of which contributes to the flavour of coffee, that are found in both raw and roasted grains. In the latter, diacylglycerols formed by the triacylglycerols during roasting are also detected. The second part focuses on the analysis of proteins on TEFLON® surface. Dried spots of proteins’ aqueous solutions on TEFLON® surface are analyzed, giving typical mass spectra. A large part of these analysis involves the selection of optimum parameters for the analysis, such as the flow rate of the nebulizer solvent and the backpressure of the gas. Additives (HCOOH, DMSO etc.) are added either to the protein solutions or nebulizer solvent and their effect on the mass spectra is determined. The third and final part consists of the analysis of euro banknotes and US dollars. From euro banknote areas, mass spectra are obtained and these profiles are compared with other banknotes with a view of identifying counterfeit notes. US dollar analyzes indicate the presence of cocaine on their surface after identification of characteristic drug peaks by MS/MS.

Language

Greek

Subject

Bank notes

Coffee beans

Proteins

Καφές

Πρωτεϊνες

Χαρτονόμισμα

Issue date

2019-03-27

Collection