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Identifier

000363516

Title

In vivo ανάλυση της φυτικής 3'-5' εξωνουκλεάσης ERL1

Alternative Title

In vivo analyses of plant 3'-5' exonuclease ERL1

Author

Βαμβακά, Ευγενία

Thesis advisor

Καλαντίδης, Κρίτων
Τσαγρή, Ευθυμία
Κοτζαμπάσης, Κωνσταντίνος

Abstract

Chloroplasts are subcellular organelles combine features from prokaryotic organisms, because of their origin from symbiotic photosynthetic bacteria, and also from eukaryotes, due to their adaptation in such environment. In plants chloroplasts possess their own genome, which during endosymbiosis, lost its genetic autonomy and many genes transferred to the nucleus. Nevertheless some genes remained at chloroplasts and these genes encode components of photosynthetic and transcription-translation apparatus of the plastids. The control of gene expression in plastids is a post-transcriptional process with RNA splicing, turnover, editing and processing being the primarily procedures. RNA processing includes cleavage of polycistronic transcripts and also the maturation of 5’ and 3’ ends of them. Endonucleolytic and exonucleolytic mechanisms participate in mature ends formation of transcripts. For mature 3’ ends responsible are 3’-5’ exonucleases, such as PNPase and RNase R1, which are parts of ribosomal RNA metabolism. This category includes also DEDD exonucleases, part of them are the ERI1s, which use as substrates double stranded DNA /RNA molecules and catalyze their single stranded 3’ ends. ERI1s participate in pathways of RNA silencing and processing of rRNAs in many organisms. In plants it seems that ERL1 do not play any role in RNAi, but participate in rRNA processing, 5S specifically. The initial query of this thesis was to clone ERL1 from N.benthamiana and A.thaliana in order to overexpress and downregulate it. The plasmid was checked for their functionality. Afterwards specific chloroplastic genes have been checked for differences in expression levels. Simultaneously we produced transgenic plants N.benthamiana with ERL1 Hairpin and try to establish their functionality. The aim was the use of them in chloroplastic genes analyses. Finally, transgenic plants A.thaliana were grown and analyzed for the study of the same genes and the comparison of them between these two plant species.

Language

Greek

Subject

Chloroplast metabolish

DEDDh family

Post-transcriptional processes

RNA processing

RNA επεξεργασία

Επεξεργασία ριβοσωμικού RNA

Οικογένεια DEDDh

Χλωροπλαστικός μεταβολισμός

Issue date