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Identifier	uch.biology.msc//2001kotsis
Title	Χρήση της μετα-μεταγραφικής σίγησης γονιδίων για τη δημιουργία διαγονιδιακών φυτών ανθεκτικών σε φυτικούς ιούς
Author	Κότσης, Δημήτριος
Thesis advisor	Τσαγρή, Ευθυμία
Abstract	Posttranscriptional gene silencing (PTGS) and RNA interference are different versions of an ancient mechanism, which was developed in organisms in order to regulate development and/or combat invading viruses and transposons. In PTGS, RNAs with specific sequence are targeted and degraded by a mechanism which is currently under investigation. A central role in this cascade of events which leads to the RNA degradation is played by double stranded RNA molecules (ds RNA). Expression of dsRNA of a specific gene in a plant, leads to degradation of its homologous RNA, independently whether this is a viral or host gene. We have used the dsRNA strategy in order to produce virus resistant plants.We have produced dsRNA genes in plant transformation vectors for PPV, PVX, PVY viruses. Transgenic Nicotiana benthamiana plants have been produced, which contain the dsRNA genes for PPV and PVX. Also, transgenic Nicotiana benthamiana plants expressing (+) PPV RNA only have been produced. ds and (+)PPV RNA plants of the T0 generation have been challenged with PPV virus. The dsRNA producing plant lines were at a high percentage resistant to the virus, while, from the (+) RNA lines tested, none line was found resistant.These results indicate, that the dsRNA strategy can be successfully applied to crop plants, and thus help to control important virus diseases.
Language	Greek
Issue date	2001-12-06
Collection	School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses
	Type of Work--Post-graduate theses
Permanent Link	https://elocus.lib.uoc.gr//dlib/5/0/9/metadata-dlib-2001kotsis.tkl
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