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Identifier	000431679
Title	Διερεύνηση υπογραφών έκφρασης miRNA προς διαστρωμάτωση ασθενών με γλοιοβλάστωμα
Alternative Title	Deciphering miRNA signatures toward patient stratification in glioblastoma multiforme
Author	Μπαφίτη, Παρασκευή
Thesis advisor	Κατσίλα, Θεοδώρα
Reviewer	Πίντζας, Αλέξανδρος Τσατσάνης, Χρήστος
Abstract	Malignant gliomas constitute a complex disease phenotype that demands optimum decision-making. Despite being the most common type of primary brain tumors, gliomas are highly heterogeneous when their pathophysiology and response to treatment are considered. Such inter-individual variability also renders optimum patient stratification extremely difficult. Recent evidence highlight that the gene-environment interplay becomes of fundamental importance in oncogenesis and progression of gliomas. For glioblastoma multiforme, in particular, genotype-to-phenotype associations are critical. Herein, we put emphasis on a microRNA signature (hsa-miR-20a, hsa-miR-21, hsa-miR-10a) to map the inter-individual variability of glioblastoma multiforme patients toward optimum patient stratification. Data and text mining reported a key role for hsa-miR-20a, hsa-miR-21 and hsa-miR-10a in the regulation of gene expression in glioblastoma pathophysiology. Next, immunohistochemistry analysis of FFPE tissue samples of glioblastoma multiforme patients (n=37) was performed for TGFb-RII, hMSH2 and EphA8. microRNA expression levels were determined by RT-qPCR. hsa-miR-20a, hsa-miR-21 and hsa-miR-10a were up-regulated. Such findings are further supported by our immunohistochemistry analysis for TGFb-RII, hMSH2 and EphA8. Molecular-clinical correlations were drawn to classify high- vs. low-risk patients, shedding light to the molecular mechanisms involved in disease progression. This microRNA signature (hsa-miR-20a, hsa-miR-21, hsa-miR-10a) may further empower evidence-informed decision-making in clinical practice.
Language	Greek, English
Subject	Metabotype
	Μεταβότυπος
Issue date	2020-08-05
Collection	School/Department--School of Medicine--Department of Medicine--Post-graduate theses
	Type of Work--Post-graduate theses
Permanent Link	https://elocus.lib.uoc.gr//dlib/c/6/3/metadata-dlib-1598960794-365343-29358.tkl
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