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Identifier 000456740
Title Βιοτεχνολογική καταπολέμηση του δάκου της ελιάς (Bactrocera oleae) με το βακτήριο Wolbachia
Alternative Title Wolbachia as a tool for biotecnological control of Bactrocera oleae
Author Μουστάκα, Ιουλιέττα Μ.
Thesis advisor Βόντας, Ιωάννης
Reviewer Κοτζαμπάσης, Κυριάκος
Καλαντίδης, Κρίτων
Abstract Olea europaea is one of the most economically important tree crops in Greece and the Mediterranean basin that is known for its nutritional importance in the Mediterranean diet. The olive fruit fly, Bactrocera oleae, is considered the most serious pest of olives. Its endosymbiotic bacteria Erwinia dacicola is required by B. oleae in order to complete its development in unripe green olives. Several control strategies have been used against B. oleae such as chemical insecticide treatments, sterile insect technique (SIT) and the use of traps and pheromones. The purpose of this work was to investigate the potential of Wolbachia, another obligatory endosymbiotic bacterium, which has already been used in biotechnological control applications of several insects, to control B. oleae. Specifically, I set two different goals, firstly, the investigation of the possible interaction between Wolbachia with Erwinia dacicola, and secondly, the ability of Wolbachia to induce cytoplacmic incompatibility (CI) in its hosts. Firstly, we confirmed the maternal transmission of Erwinia and then we localized the bacteria in the esophageal bulb of adults with PCR, fluorescence microscopy and confocal microscopy and we estimated the number of bacteria per bulb at 2.5 million with qPCR. Subsequently we investigated means for the infection of the lab populations with Erwinia dacicola. We found that the injections of the esophageal bulb containing E. dacicola to the head of uninfected females is optimal. We created a population with both E. dacicola and Wolbachia in order to οbserve any possible interactions. Simultaneously, we searched out for new Wolbachia populations adapted to the enviroment of Crete, which will be ideal for the application of the control of B. oleae in Crete. We identified two different Wolbachia strains, the wRi and wMel, and we proceeded in the creation of new lab populations with these two Wolbachia strains, but without identifying any bacteria transmission.
Language Greek
Issue date 2017-03-17
Collection   School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses
  Type of Work--Post-graduate theses
Permanent Link https://elocus.lib.uoc.gr//dlib/5/e/d/metadata-dlib-1687947493-956632-32255.tkl Bookmark and Share
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