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Identifier

000404252

Title

Structural evaluation of animal ocular models by means of photoacoustic microscopy

Alternative Title

Εκτίμηση της δομής μοντέλων ζωικών οφθαλμών μέσω οπτοακουστικής μικροσκοπίας

Author

Αβτζή, Στυλιανή

Thesis advisor

Τσιλιμπάρης, Μιλτιάδης

Reviewer

Ζαχαράκης, Γιάννης
Τσερεβελάκης, Γιώργος

Abstract

Photoacoustic imaging is a rapidly developing new biomedical imaging modality. The previous years photoacoustic imaging has been used to measure brain metabolism1, for microvasculature and lymph nodes imaging2, for melanoma detection3, breast cancer4, to measure oxygenation5 etc. Unlike existing ophthalmic imaging technologies photoacoustic imaging combines the capabilities of optical excitation and acoustic detection. Light absorbing biomolecules such as melanin and hemoglobin can provide intrinsic imaging contrast at high sensitivity. In this work, the feasibility of hybrid photoacoustic and optical imaging of two major molecular contrast agents, melanin and lipofuscin, in ocular models was investigated. Imaging was based on two physical processes depending on photon absorption: opto(photo)acoustic effect and autofluorescence. The aim of this work was to obtain structural and molecular information of the ciliary body of healthy rabbit eyes. A series of experiments were performed including the imaging ranging from small parts to whole uncut ciliary bodies. photooacoustic signal was primarily generated due to the absorption of melanin that lies on the RPE cells of the ciliary body, while autofluorescence was mainly emitted by lipofuscin depositions. As a result, this thesis is an effort to test the capabilities of a prototype hybrid microscopy system as to the high resolution label-free imaging of ocular models, constituting a stepping stone for further application potential investigation in opthalmological research.

Language

English

Subject

Biomedical imaging

Ακτινωτό σώμα

Βιοϊατρική απεικόνηση

Issue date

2016 -12-13

Collection

School/Department--School of Medicine--Department of Medicine--Post-graduate theses

Type of Work--Post-graduate theses

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