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Identifier 000326704
Title Ανάλυση μκροσυστοιχιών και χρήση της ποσοτικής Real-time PCR με σκοπό: Α. Τη μελέτη του προτύπου έκφρασης του γονιδίου Gtl2 στο νωτιαίο μυελό των μυών Β. Τη μελέτη της έκφρασης γονιδίων στους TAG-1-/- μύες
Author Ζούπη, Λήδα
Thesis advisor Καραγωγέως, Δόμνα
Καλαντίδης, Κρίτων
Abstract Until recently, approximately 70 genetically imprinted genes have been identified, many of which are thought to play a role in neurodevelopment and behavior. In order to elucidate the role of specific genes in these processes, the observation of their expression pattern in the central nervous system is required. Preliminary data from a microarray study have revealed the differential expression of Gtl2, a maternally expressed non-coding RNA, between the ventral and the dorsal telencephalon of the mouse. Moreover, a series of in situ hybridizations in coronal brain sections from mice indicated a very interesting expression pattern in different parts of the brain. In order to obtain an overall image of the Gtl2 expression pattern we performed in situ hybridization in transverse sections of mouse spinal cord at different developmental stages (E11.5 to P7). Strong expression was observed in the cell bodies of the gray matter and in the dorsal root ganglia. TAG-1 is a neural specific cell adhesion molecule of the immunoglobulin superfamily, with a strong expression in different parts of the central and peripheral nervous system in rodents. This protein is thought to be implicated in fasciculation, neurite outgrowth, axonal migration and myelination events. The mechanisms through which it acts and the overall number of molecules which are able to interact with TAG-1, are still to be elucidated. In this study, through the use of microarray analysis and of quantitative Real time PCR, we aim to reveal new interacting molecules and possible downstream signaling events in which our protein is implicated.
Language Greek
Issue date 2008-04-07
Collection   Faculty/Department--School of Medicine--Department of Medicine--Post-graduate theses
  Faculty/Department--Faculty of Sciences and Engineering--Department of Biology--Post-graduate theses
  Type of Work--Post-graduate theses
Notes Διατμηματικό πρόγραμμα μεταπτυχιακών σπουδών:" Μοριακή Βιολογία - Βιοϊατρική"
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