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Identifier

000395197

Title

Σύνθεση και μελέτη φθορισμομετρικού προφίλ θειούχων άκυκλων δεικτών ιόντων υδραργύρου

Alternative Title

Synthesis and fluorimetric profile study of acyclic sulphide indicators for mercury ions

Author

Τσικριτζή, Αθανασία Γ

Thesis advisor

Κατερινόπουλος, Χαράλαμπος Ε.

Reviewer

Ορφανόπουλος, Μιχαήλ
Στρατάκης, Εμμανούλ

Abstract

This research project involves the synthesis and spectral profile study of four fluorometric indicators with specificity for heavy metals. The synthesized sensors are compounds 7, 10, 12 and 13, with the following structures: Ion-selectivity studies of these sensors in the presence of various biological metal ions such as K⁺, Na⁺, Pd²⁺, Cd²⁺, Zn²⁺, Ca²⁺, Cu²⁺, Fe²⁺ indicated that sensor 10 displays greater selectivity for divalent mercury (Hg²⁺) which was the main target ion in this work. Sensor 7 shows no selectivity for Hg²⁺ ions and behaves as “turn-off”, with excitation and emission wavelength maxima at λ_exc: 335 nm and λ_em: 395 nm, respectively, and a dissociation constant value K_d = 37.58 μM for Hg²⁺. Sensor 10, in the presence of increasing Hg²⁺ ion concentrations, exhibits an increase in relative fluorescence intensity, i.e. a “turn-on” behavior, with excitation and emission maxima at λ_exc: 337 nm and λ_em: 409 nm, respectively, and a dissociation constant value of K_d = 10.17 μM for Hg²⁺. In the presence of the rest biological ions compound 10 functions as a “turn-off” sensor. Sensor 12 exhibits a positive response to the presence of Hg²⁺ ions (“turn-on”) with excitation and emission maxima at λ_exc: 337 nm and λ_em: 407 nm, respectively, and a dissociation constant value of K_d = 22.33 μM. Finally, sensor 13, in the presence of increasing Hg²⁺ ion concentrations, exhibits an increase in relative fluorescence intensity (“turn-on”), with excitation and emission maxima at λ_exc: 342 nm and λ_em: 412 nm, respectively, and a dissociation constant value of K_d = 179.70mM. In conclusion, among the four sensors studied, the most promising indicator for measuring Hg²⁺ ions in biological systems is sensor 10.

Language

Greek, English

Subject

Fluorescent techniques

Heavy metal

Ions sensors

Βαρέα μέταλλα

Δείκτες Ιόντων

Φθορισμομετρικές Τεχνικές

Issue date

2015-07-17

Collection