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| Identifier |
000387988 |
| Title |
Ανάπτυξη άμεσων αμπερομετρικών βιοαισθητήρων βασισμένων σε αναδιπλούμενες πρωτεΐνες δέσμευσης |
| Alternative Title |
Development of amperometric reagentless biosensors based on hinge-motion binding proteins |
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Author
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Τσεμπερούλη, Μαρία Δημήτριος
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Thesis advisor
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Χανιωτάκης, Νικόλαος
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Reviewer
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Γανωτάκης, Δημήτριος
Σπύρος, Απόστολος
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| Abstract |
Electrochemical biosensors provide an attractive means for the qualitative characterization and quantification for a diverse set of analytes. The extensive research in this field the last 50 years is focused on the optimization of their analytical performance and overcoming their operational problems. Some of the major obstacles in the biosensor systems include their low sensitivity and the poor sensor to sensor reproducibility. For this reason, the scientific interest in this field has focused on the simplification of the elaborate techniques required for the electrochemical biosensor development. This can be realized by exploiting the intrinsic physicochemical and biochemical properties of biological macromolecules, and by using signal transducers with optimized or unique electrochemical characteristics.
The aim of this work is the development of reagentless amperometric biosensors for transducing ligand binding events of ligand binding proteins. The basic characteristic of this superfamily of proteins is their hinge motion upon ligand binding. The method, is based on exploiting the induced large conformational changes of protein structure upon their ligand binding, and transducing this change into electrochemical response by using an electrochemical signal transducer. For the development of method presented here, Calmodulin was used as ligand binding protein. Calmodulin (CaM), a small calcium modulated protein, is an ubiquitous Ca2+- sensor in all eukaryotic cells and undergoes a large conformational change upon the binding of four calcium ions.
Calomodulin based biosensor was developed by adsorption of protein on a screen printed electrode surface and its electrochemical performance was evaluated by electrochemical methods: chronoamperometry and electrochemical impedance spectroscopy. The results obtained by testing the response of Calmodulin based biosensor in the presence of Ca2+, set the basis for the development of new bioelectronic interfaces for the reagentless and selective detection of analytes for defense applications.
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| Language |
Greek |
| Issue date |
2014-11-21 |
| Collection
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School/Department--School of Sciences and Engineering--Department of Chemistry--Post-graduate theses
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Type of Work--Post-graduate theses
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| Permanent Link |
https://elocus.lib.uoc.gr//dlib/0/b/a/metadata-dlib-1415002860-991573-20416.tkl
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| Views |
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